Detailed information of TA system TA234636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3329448..3330153
• Replicon: chromosome
• Accession: NZ_CP091704
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: L6L59_RS16220
• Protein ID: WP_000539521.1
• Coordinates: 3329448..3329834 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L59_RS16225
• Protein ID: WP_001280945.1
• Coordinates: 3329824..3330153 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS16200 (3325452)	3325452..3326078	+	627	WP_001295292.1	glutathione S-transferase GstB	-
L6L59_RS16205 (3326075)	3326075..3327190	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
L6L59_RS16210 (3327301)	3327301..3327684	-	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L59_RS16215 (3327897)	3327897..3329222	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L59_RS16220 (3329448)	3329448..3329834	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L59_RS16225 (3329824)	3329824..3330153	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L59_RS16230 (3330223)	3330223..3331551	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
L6L59_RS16235 (3331559)	3331559..3333907	-	2349	WP_001550886.1	EAL domain-containing protein	-
L6L59_RS16240 (3334085)	3334085..3334996	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T234636 WP_000539521.1 NZ_CP091704:3329448-3329834 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234636 NZ_LR133964:731687-732172 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234636 WP_001280945.1 NZ_CP091704:3329824-3330153 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234636 NZ_LR133964:731397-731690 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6