Detailed information of TA system TA234635

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3063419..3064203
• Replicon: chromosome
• Accession: NZ_CP091704
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: L6L59_RS14940
• Protein ID: WP_000613626.1
• Coordinates: 3063709..3064203 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: L6L59_RS14935
• Protein ID: WP_001110447.1
• Coordinates: 3063419..3063712 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS14925 (3058569)	3058569..3059528	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
L6L59_RS14930 (3060101)	3060101..3063286	+	3186	WP_001550951.1	ribonuclease E	-
L6L59_RS14935 (3063419)	3063419..3063712	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
L6L59_RS14940 (3063709)	3063709..3064203	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
L6L59_RS14945 (3064298)	3064298..3065251	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
L6L59_RS14950 (3065263)	3065263..3066906	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
L6L59_RS14955 (3066972)	3066972..3067913	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
L6L59_RS14960 (3067913)	3067913..3069010	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T234635 WP_000613626.1 NZ_CP091704:3063709-3064203 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T234635 NZ_LR133964:336946-337314 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT234635 WP_001110447.1 NZ_CP091704:3063419-3063712 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT234635 NZ_LR133964:336728-336949 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6