Detailed information of TA system TA234634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2627893..2628531
• Replicon: chromosome
• Accession: NZ_CP091704
• Organism: Escherichia coli strain KGu016

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: L6L59_RS12580
• Protein ID: WP_000813795.1
• Coordinates: 2628355..2628531 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L6L59_RS12575
• Protein ID: WP_076797675.1
• Coordinates: 2627893..2628309 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L59_RS12555 (2623045)	2623045..2623986	-	942	WP_001251315.1	ABC transporter permease	-
L6L59_RS12560 (2623987)	2623987..2625000	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
L6L59_RS12565 (2625018)	2625018..2626163	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
L6L59_RS12570 (2626408)	2626408..2627814	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
L6L59_RS12575 (2627893)	2627893..2628309	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L6L59_RS12580 (2628355)	2628355..2628531	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L6L59_RS12585 (2628753)	2628753..2628983	+	231	WP_023910283.1	YncJ family protein	-
L6L59_RS12590 (2629075)	2629075..2631036	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L6L59_RS12595 (2631109)	2631109..2631645	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
L6L59_RS12600 (2631737)	2631737..2632909	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T234634 WP_000813795.1 NZ_CP091704:c2628531-2628355 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T234634 NZ_LR133932:c5127458-5127048 [Klebsiella oxytoca]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAATGGTTCTCACTGTTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGGTGTTGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCAGACAGGGGGAAATAAAGCTGCTGATTGACTCTCGCCTGCGCTGGCAAAAAGCG
GAGTGGGATATCGTCGGCACCCCGTGGGTAATCAATAGCGGAATGCTGCTGCGCCTGAGAAATACGGAAAACCAACGCAC
TCAGCATCTATGGGTGGCGGCGGATAGCATGGACGCGGGCGAGTGGCGCGATCTGCGTCGGCTGGTGTTACAGAAACCGA
CGCAAGATTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT234634 WP_076797675.1 NZ_CP091704:c2628309-2627893 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT234634 NZ_LR133932:c5127705-5127439 [Klebsiella oxytoca]
ATGGATATTAACAATAAAGCTCGCATCCACTGGGCATGCCGCCGGGGAATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTTGAGCATGAGTACGATACGCTCAGTGATGACGATAAGCGGTTGTTCATCCGCCTGCTGGAAAATGACGATCCGGACC
TGTTCAACTGGTTGATGAACCATGGCGAACCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1