Detailed information of TA system TA023463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2857242..2858284
• Replicon: chromosome
• Accession: NC_011770
• Organism: Pseudomonas aeruginosa LESB58

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: PLES_RS13840
• Protein ID: WP_003153636.1
• Coordinates: 2857709..2858284 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: PLES_RS13835
• Protein ID: WP_003050245.1
• Coordinates: 2857242..2857712 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLES_RS13800	2852621..2854039	-	1419	WP_004350605.1	TIGR03752 family integrating conjugative element protein	-
PLES_RS13805	2854029..2854940	-	912	WP_004350604.1	TIGR03749 family integrating conjugative element protein	-
PLES_RS13810	2854937..2855629	-	693	WP_012614071.1	TIGR03746 family integrating conjugative element protein	-
PLES_RS13815	2855626..2856036	-	411	WP_003821108.1	TIGR03750 family conjugal transfer protein	-
PLES_RS13820	2856049..2856408	-	360	WP_003153634.1	TIGR03745 family integrating conjugative element membrane protein	-
PLES_RS13825	2856425..2856658	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
PLES_RS13830	2856655..2857038	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
PLES_RS13835	2857242..2857712	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
PLES_RS13840	2857709..2858284	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
PLES_RS13845	2858302..2859216	+	915	WP_003050256.1	AAA family ATPase	-
PLES_RS13850	2859213..2859683	+	471	WP_003153638.1	hypothetical protein	-
PLES_RS13855	2859680..2860180	+	501	WP_003090159.1	hypothetical protein	-
PLES_RS13860	2860180..2861082	+	903	WP_003153640.1	nucleotidyltransferase domain-containing protein	-
PLES_RS13865	2861121..2861846	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2796694..2907811	111117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T23463 WP_003153636.1 NC_011770:2857709-2858284 [Pseudomonas aeruginosa LESB58]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T23463 NC_011770:2857709-2858284 [Pseudomonas aeruginosa LESB58]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT23463 WP_003050245.1 NC_011770:2857242-2857712 [Pseudomonas aeruginosa LESB58]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT23463 NC_011770:2857242-2857712 [Pseudomonas aeruginosa LESB58]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68