Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4159950..4160604 Replicon chromosome
Accession NZ_CP091703
Organism Escherichia coli strain KOr021

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L6L60_RS19790 Protein ID WP_000244777.1
Coordinates 4160197..4160604 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L60_RS19785 Protein ID WP_000354046.1
Coordinates 4159950..4160216 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L60_RS19760 (4155119) 4155119..4155862 + 744 WP_000951964.1 SDR family oxidoreductase -
L6L60_RS19765 (4155919) 4155919..4157352 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
L6L60_RS19770 (4157397) 4157397..4157708 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
L6L60_RS19775 (4157872) 4157872..4158531 + 660 WP_000250274.1 hemolysin III family protein -
L6L60_RS19780 (4158727) 4158727..4159707 - 981 WP_000886083.1 tRNA-modifying protein YgfZ -
L6L60_RS19785 (4159950) 4159950..4160216 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L60_RS19790 (4160197) 4160197..4160604 + 408 WP_000244777.1 protein YgfX Toxin
L6L60_RS19795 (4160644) 4160644..4161165 - 522 WP_001055874.1 flavodoxin FldB -
L6L60_RS19800 (4161277) 4161277..4162173 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L60_RS19805 (4162198) 4162198..4162908 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L60_RS19810 (4162914) 4162914..4164647 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234614 WP_000244777.1 NZ_CP091703:4160197-4160604 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234614 NZ_LR130759:c4619259-4618822 [Mycobacterium basiliense]
ATGGCGAAACTCTCCGGATCGATCGATGTCCCGTTGGCCCCGGAGGAGGCATGGCAGCACGCCTCCGATCTGTCCCGCTA
CAAGGACTGGCTGACCATCCACCGCGTGTGGCGCAGCACGTTGCCCGAATCCCTGGAGAAGGGCGCGGTCGTCGAATCGA
TCGTCCAGGTCAAAGGCATGCACAACCGGATCAAGTGGACGATCGTGCGCTACAAGCCGCCGGAAGGAATGACCCTCAAC
GGAGACGGTGTGGGCGGCGTCAAAGTCAAGCTCATGGCCAAGGTCGCGCCCAAGGAAGACGGCTCGGTGGTCAGCTTCGA
TGTGCACCTGGGCGGGCCCGCCCTGTTCGGGCCGATCGGCATGATCGTCGCCGGTGCTCTGAGAAGCGATATCCGCGAAT
CGCTGGAAAACTTCGTCACCGTGTTCGCGCCGGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234614 WP_000354046.1 NZ_CP091703:4159950-4160216 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234614 NZ_LR130759:c4619452-4619264 [Mycobacterium basiliense]
ATGGGAATCCTGGACAAGGCAAAGAACCTGCTGTCGCAAAACGCCGACAAGGTCGAGACAGCGATCGACAAGGCCGGCGA
GTTCGTCGACCAAAAGACGCAGGGCAAGTATTCCGACACCATCCACAAGGTGCAGGAAGAGGCCAAGAAAGTCGCTGGCC
CCGGTGCCGCTGGCGACCAACAGCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References