Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4026810..4027645 Replicon chromosome
Accession NZ_CP091703
Organism Escherichia coli strain KOr021

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0A1A9A5
Locus tag L6L60_RS19090 Protein ID WP_001564063.1
Coordinates 4026810..4027187 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0A1AEL8
Locus tag L6L60_RS19095 Protein ID WP_038432125.1
Coordinates 4027277..4027645 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L60_RS19060 (4021938) 4021938..4023086 - 1149 WP_000905920.1 capsule polysaccharide export inner-membrane protein KpsE -
L6L60_RS19065 (4023158) 4023158..4024141 - 984 WP_001361242.1 KpsF/GutQ family sugar-phosphate isomerase -
L6L60_RS19070 (4024952) 4024952..4025122 - 171 Protein_3720 IS110 family transposase -
L6L60_RS19075 (4025464) 4025464..4026306 - 843 Protein_3721 DUF4942 domain-containing protein -
L6L60_RS19080 (4026391) 4026391..4026585 - 195 WP_024181941.1 DUF957 domain-containing protein -
L6L60_RS19085 (4026664) 4026664..4026813 - 150 Protein_3723 DUF5983 family protein -
L6L60_RS19090 (4026810) 4026810..4027187 - 378 WP_001564063.1 TA system toxin CbtA family protein Toxin
L6L60_RS19095 (4027277) 4027277..4027645 - 369 WP_038432125.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
L6L60_RS19100 (4027808) 4027808..4028029 - 222 WP_000692345.1 DUF987 domain-containing protein -
L6L60_RS19105 (4028094) 4028094..4028570 - 477 WP_021553055.1 RadC family protein -
L6L60_RS19110 (4028586) 4028586..4029065 - 480 WP_001564060.1 antirestriction protein -
L6L60_RS19115 (4029331) 4029331..4030149 - 819 WP_001175165.1 DUF932 domain-containing protein -
L6L60_RS19120 (4030239) 4030239..4030472 - 234 WP_001278293.1 DUF905 family protein -
L6L60_RS19125 (4030478) 4030478..4031155 - 678 WP_001564058.1 hypothetical protein -
L6L60_RS19130 (4031306) 4031306..4031986 - 681 WP_001278649.1 WYL domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 4025337..4090598 65261
- flank IS/Tn - - 4024952..4025056 104


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14094.09 Da        Isoelectric Point: 7.8045

>T234613 WP_001564063.1 NZ_CP091703:c4027187-4026810 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T234613 NZ_LR130565:c129022-128717 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13569.31 Da        Isoelectric Point: 7.0369

>AT234613 WP_038432125.1 NZ_CP091703:c4027645-4027277 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT234613 NZ_LR130565:c129242-129024 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A1A9A5


Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEL8

References