Detailed information of TA system TA023461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1450939..1451596
• Replicon: chromosome
• Accession: NC_011770
• Organism: Pseudomonas aeruginosa LESB58

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8G3IXE4
• Locus tag: PLES_RS06960
• Protein ID: WP_003098540.1
• Coordinates: 1451414..1451596 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PLES_RS06955
• Protein ID: WP_004351284.1
• Coordinates: 1450939..1451367 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLES_RS06920	1446952..1447656	+	705	WP_012613696.1	phage regulatory protein/antirepressor Ant	-
PLES_RS06925	1447721..1448554	+	834	WP_012613697.1	helix-turn-helix domain-containing protein	-
PLES_RS06930	1448541..1449338	+	798	WP_012613698.1	hypothetical protein	-
PLES_RS06935	1449335..1449541	+	207	WP_024082445.1	hypothetical protein	-
PLES_RS06940	1449538..1450119	+	582	WP_012613699.1	recombination protein NinG	-
PLES_RS06945	1450116..1450412	+	297	WP_024082446.1	hypothetical protein	-
PLES_RS06950	1450414..1450788	+	375	WP_004351286.1	hypothetical protein	-
PLES_RS06955	1450939..1451367	-	429	WP_004351284.1	hypothetical protein	Antitoxin
PLES_RS06960	1451414..1451596	-	183	WP_003098540.1	type II toxin-antitoxin system HicA family toxin	Toxin
PLES_RS06965	1451797..1452111	+	315	WP_015649339.1	phage holin, lambda family	-
PLES_RS06970	1452111..1452728	+	618	WP_012613700.1	glycoside hydrolase family 19 protein	-
PLES_RS06975	1452728..1453198	+	471	WP_025208377.1	lysis system i-spanin subunit Rz	-
PLES_RS06980	1453195..1453938	+	744	WP_012613702.1	hypothetical protein	-
PLES_RS06985	1454058..1454603	+	546	WP_019486501.1	terminase small subunit	-
PLES_RS06990	1454575..1456539	+	1965	WP_012613704.1	phage terminase large subunit family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1432804..1476402	43598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-78)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6746.86 Da
• Isoelectric Point: 11.0775

>T23461 WP_003098540.1 NC_011770:c1451596-1451414 [Pseudomonas aeruginosa LESB58]
MKFSEFRRWLKAQGVTFEAGKGSHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGLK

Nucleotide

• Length: 183 bp

>T23461 NC_011770:c1451596-1451414 [Pseudomonas aeruginosa LESB58]
ATGAAGTTCAGCGAATTCAGACGATGGTTGAAGGCCCAAGGGGTGACCTTCGAAGCCGGCAAAGGGAGCCACTTCAAGAT
CACCGCCCCGAACGGCAAACAGACCACCTTCGCGGACCACGGAGCTAAGGAAATGCCAGAACCGACCCGCAAGGCGATCA
TCAAGCAACTGGGGCTCAAATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15756.94 Da
• Isoelectric Point: 4.6562

>AT23461 WP_004351284.1 NC_011770:c1451367-1450939 [Pseudomonas aeruginosa LESB58]
MYDYAIRFEQDDSAPGVAVFCRDLPELNSYGDDKVHAIGEAVDAIESTLSLYVDQRREIPAASQAQPGERVIHLPAVTVA
KIALWNEMVRRDMRKADLCRLLGIAQTQGDRLVDFLHNTKMEAMENALSALGLRLSVNIEAA

Nucleotide

• Length: 429 bp

>AT23461 NC_011770:c1451367-1450939 [Pseudomonas aeruginosa LESB58]
ATGTACGACTATGCAATCCGTTTCGAACAGGACGATAGCGCTCCTGGCGTTGCCGTTTTCTGCAGAGACTTGCCGGAGCT
GAACAGCTATGGCGACGACAAGGTCCACGCAATCGGCGAGGCAGTCGACGCCATCGAGTCGACCCTCTCGCTATACGTTG
ATCAGCGCCGAGAAATCCCCGCGGCCAGCCAGGCGCAACCAGGCGAGCGCGTTATCCATCTGCCGGCAGTTACCGTTGCG
AAGATCGCGCTCTGGAACGAAATGGTCCGTCGAGATATGCGAAAGGCTGACCTCTGCCGGCTTCTCGGGATCGCACAGAC
CCAGGGCGACAGGCTCGTCGACTTCCTCCACAACACTAAGATGGAGGCCATGGAGAACGCTCTATCCGCCCTCGGACTCC
GCCTCTCAGTGAATATCGAGGCAGCATGA