Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 2566811..2567447 Replicon chromosome
Accession NC_011761
Organism Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)


Gene name vapC Uniprot ID B7J980
Locus tag AFE_RS13250 Protein ID WP_012537455.1
Coordinates 2566811..2567227 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID B7J981
Locus tag AFE_RS13255 Protein ID WP_009560865.1
Coordinates 2567214..2567447 (-) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFE_RS15850 2561991..2562143 - 153 WP_009566804.1 integrase core domain-containing protein -
AFE_RS15395 2562150..2562437 - 288 WP_012537449.1 DDE-type integrase/transposase/recombinase -
AFE_RS13230 2562800..2563726 - 927 WP_012537450.1 uracil-DNA glycosylase -
AFE_RS13235 2563893..2564963 + 1071 WP_012537451.1 linear amide C-N hydrolase -
AFE_RS15855 2565176..2565550 - 375 WP_196491168.1 type II toxin-antitoxin system PemK/MazF family toxin -
AFE_RS13240 2565556..2565807 - 252 WP_012537453.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
AFE_RS16255 2566127..2566708 + 582 WP_146235847.1 hypothetical protein -
AFE_RS13250 2566811..2567227 - 417 WP_012537455.1 PIN domain-containing protein Toxin
AFE_RS13255 2567214..2567447 - 234 WP_009560865.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AFE_RS13260 2567446..2567706 + 261 WP_012607558.1 hypothetical protein -
AFE_RS13265 2567803..2568153 + 351 WP_012537457.1 DUF4870 domain-containing protein -
AFE_RS13270 2568250..2570112 + 1863 WP_012537458.1 ATP-binding cassette domain-containing protein -
AFE_RS13275 2570109..2570432 - 324 WP_012537459.1 hypothetical protein -
AFE_RS13280 2570425..2570736 - 312 WP_009566855.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-119)

Antitoxin

(5-37)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15358.75 Da        Isoelectric Point: 5.1352

>T23458 WP_012537455.1 NC_011761:c2567227-2566811 [Acidithiobacillus ferrooxidans ATCC 23270]
MASGKAFFDTNVLLYLLSADTAKADVAEGIMVNGGVISAQVLNEFASVAQRKMGMSFLEIREVLTPIHRICDVAHLTLDT
HIHGLRIAERYKYNVWDAMIVASALLADCAVLYTEDMHHGQILEDRLQLINPFLRADE

Download         Length: 417 bp

>T23458 NC_011761:c2567227-2566811 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGCGAGCGGTAAGGCGTTCTTCGACACGAACGTGCTGCTTTATCTGCTTTCGGCGGACACGGCCAAGGCAGATGTTGC
AGAAGGCATCATGGTGAATGGTGGTGTGATCAGTGCCCAGGTGTTGAACGAATTTGCTTCCGTTGCCCAGCGCAAGATGG
GCATGTCTTTTCTGGAAATCAGAGAGGTGCTGACCCCCATTCATCGAATTTGCGATGTCGCGCATCTTACCCTGGATACG
CACATTCATGGCCTCAGGATTGCGGAGCGATACAAATACAATGTTTGGGATGCAATGATTGTCGCATCGGCCCTGTTGGC
TGATTGCGCCGTTCTTTACACGGAGGATATGCACCACGGCCAGATCCTCGAAGACCGCTTGCAACTGATCAATCCATTTT
TGCGGGCCGACGAGTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8919.15 Da        Isoelectric Point: 8.7371

>AT23458 WP_009560865.1 NC_011761:c2567447-2567214 [Acidithiobacillus ferrooxidans ATCC 23270]
MQVSKWGNSLAVRIPTSVVEALGIKEGDDIEVEVVGERRFQIQRTPQAEELLARLRRYRGRLPADFRFDRLEAHGER

Download         Length: 234 bp

>AT23458 NC_011761:c2567447-2567214 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGCAGGTATCCAAGTGGGGCAACAGTCTGGCCGTCCGTATACCAACCTCCGTGGTAGAGGCGCTGGGTATCAAAGAGGG
TGACGACATTGAGGTTGAGGTCGTGGGCGAACGCCGTTTTCAGATTCAACGAACGCCTCAAGCGGAAGAGTTGTTGGCCA
GGTTAAGGCGTTATCGAGGGAGGCTCCCTGCCGATTTCAGGTTCGACCGGTTGGAAGCGCATGGCGAGCGGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB B7J980


Antitoxin

Source ID Structure
AlphaFold DB B7J981

References