Detailed information of TA system TA234576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2237148..2237786
• Replicon: chromosome
• Accession: NZ_CP091700
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: L6L55_RS11805
• Protein ID: WP_000813794.1
• Coordinates: 2237148..2237324 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L6L55_RS11810
• Protein ID: WP_001270286.1
• Coordinates: 2237370..2237786 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS11785 (2232767)	2232767..2233981	-	1215	WP_096977194.1	BenE family transporter YdcO	-
L6L55_RS11790 (2234034)	2234034..2234570	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
L6L55_RS11795 (2234643)	2234643..2236604	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L6L55_RS11800 (2236696)	2236696..2236926	-	231	WP_000494244.1	YncJ family protein	-
L6L55_RS11805 (2237148)	2237148..2237324	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L6L55_RS11810 (2237370)	2237370..2237786	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L6L55_RS11815 (2237865)	2237865..2239271	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
L6L55_RS11820 (2239516)	2239516..2240661	+	1146	WP_214142448.1	ABC transporter substrate-binding protein	-
L6L55_RS11825 (2240679)	2240679..2241692	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
L6L55_RS11830 (2241693)	2241693..2242634	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T234576 WP_000813794.1 NZ_CP091700:2237148-2237324 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T234576 NZ_LR130562:c2648296-2648120 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT234576 WP_001270286.1 NZ_CP091700:2237370-2237786 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT234576 NZ_LR130562:c2648074-2647658 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB