Detailed information of TA system TA023457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1882734..1883371
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFE_RS09815
• Protein ID: WP_012536995.1
• Coordinates: 1882734..1883144 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7J4Y7
• Locus tag: AFE_RS09820
• Protein ID: WP_009567455.1
• Coordinates: 1883141..1883371 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS09795	1879314..1880357	-	1044	WP_012607324.1	phytoene/squalene synthase family protein	-
AFE_RS09800	1880516..1881550	-	1035	WP_012536992.1	NAD(P)-dependent glycerol-3-phosphate dehydrogenase	-
AFE_RS09805	1881554..1882003	-	450	WP_012536993.1	protein-export chaperone SecB	-
AFE_RS09815	1882734..1883144	-	411	WP_012536995.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AFE_RS09820	1883141..1883371	-	231	WP_009567455.1	antitoxin	Antitoxin
AFE_RS09825	1883674..1885194	-	1521	WP_012536996.1	aldehyde dehydrogenase	-
AFE_RS09830	1885497..1887209	+	1713	WP_009566247.1	proline--tRNA ligase	-
AFE_RS09835	1887202..1887678	+	477	WP_012536997.1	universal stress protein	-
AFE_RS09840	1887691..1888356	-	666	WP_012536998.1	membrane integrity-associated transporter subunit PqiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15375.79 Da
• Isoelectric Point: 8.0756

>T23457 WP_012536995.1 NC_011761:c1883144-1882734 [Acidithiobacillus ferrooxidans ATCC 23270]
VIKYLLDTNICIFTIKNKPPEIREQFRQHHGQMAISTVTLMELIYGAEKSQVPERNLAEVEGFAARLEVLEFGSEAAKHT
GQLRAELAKIGKPIGPYDQMIAGHARSLGLFVVTNNLREFERVPGLRVMDWQSSRL

Nucleotide

• Length: 411 bp

>T23457 NC_011761:c1883144-1882734 [Acidithiobacillus ferrooxidans ATCC 23270]
GTGATCAAATACCTGCTGGATACCAATATCTGCATCTTTACTATCAAGAACAAGCCACCGGAGATCCGCGAACAGTTCCG
TCAGCACCATGGGCAAATGGCTATCAGCACCGTCACCTTGATGGAGTTGATCTATGGCGCGGAGAAATCACAGGTACCGG
AACGCAACCTGGCCGAAGTTGAAGGGTTCGCCGCCAGGCTGGAAGTGCTCGAATTCGGGAGCGAAGCCGCCAAGCATACT
GGGCAGCTCCGCGCAGAACTGGCCAAGATCGGCAAACCCATCGGGCCTTATGACCAGATGATCGCAGGCCACGCCAGAAG
CCTGGGATTGTTTGTCGTGACCAACAACTTGCGGGAATTCGAGCGCGTTCCGGGATTGCGGGTCATGGATTGGCAGAGCT
CACGCCTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8573.77 Da
• Isoelectric Point: 5.0508

>AT23457 WP_009567455.1 NC_011761:c1883371-1883141 [Acidithiobacillus ferrooxidans ATCC 23270]
MEHATVFQINRSQAIRLPKSIAFPADVKRVEVVAIGRMRILVPAGESWESWFDGEGVSADFMESREQPAGQFREPL

Nucleotide

• Length: 231 bp

>AT23457 NC_011761:c1883371-1883141 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGAGCATGCCACTGTTTTTCAGATCAACCGTAGCCAGGCGATACGTCTGCCCAAGTCGATAGCCTTTCCGGCAGATGT
GAAGCGCGTAGAGGTGGTCGCCATAGGCCGCATGCGCATTCTGGTTCCTGCCGGAGAGTCCTGGGAGAGCTGGTTCGATG
GAGAAGGGGTCAGCGCCGACTTCATGGAAAGCCGTGAGCAACCCGCCGGCCAGTTCCGGGAACCGTTGTGA

Structures

Antitoxin

• AlphaFold DB: B7J4Y7