Detailed information of TA system TA234567

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1572952..1573657
• Replicon: chromosome
• Accession: NZ_CP091700
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: L6L55_RS08425
• Protein ID: WP_000539521.1
• Coordinates: 1573271..1573657 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L55_RS08420
• Protein ID: WP_001280945.1
• Coordinates: 1572952..1573281 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS08405 (1568110)	1568110..1569021	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
L6L55_RS08410 (1569199)	1569199..1571546	+	2348	Protein_1458	EAL domain-containing protein	-
L6L55_RS08415 (1571554)	1571554..1572882	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
L6L55_RS08420 (1572952)	1572952..1573281	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L55_RS08425 (1573271)	1573271..1573657	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L55_RS08430 (1573883)	1573883..1575208	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L55_RS08435 (1575421)	1575421..1575804	+	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L55_RS08440 (1575915)	1575915..1577030	+	1116	WP_000554946.1	aldose sugar dehydrogenase YliI	-
L6L55_RS08445 (1577027)	1577027..1577653	-	627	WP_089622771.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T234567 WP_000539521.1 NZ_CP091700:c1573657-1573271 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234567 NZ_LR130562:c635251-634787 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234567 WP_001280945.1 NZ_CP091700:c1573281-1572952 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234567 NZ_LR130562:c635586-635251 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6