Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1143002..1143839 Replicon chromosome
Accession NZ_CP091700
Organism Escherichia coli strain KFu019

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag L6L55_RS06325 Protein ID WP_000227784.1
Coordinates 1143002..1143544 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag L6L55_RS06330 Protein ID WP_001297137.1
Coordinates 1143528..1143839 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L55_RS06305 (1138540) 1138540..1139451 - 912 WP_000705853.1 2-dehydropantoate 2-reductase -
L6L55_RS06310 (1139619) 1139619..1140110 + 492 WP_001138904.1 nucleotide binding protein YajQ -
L6L55_RS06315 (1140238) 1140238..1141602 - 1365 WP_001000978.1 MFS transporter -
L6L55_RS06320 (1142011) 1142011..1142946 + 936 WP_214142341.1 tetratricopeptide repeat protein -
L6L55_RS06325 (1143002) 1143002..1143544 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
L6L55_RS06330 (1143528) 1143528..1143839 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
L6L55_RS06335 (1144024) 1144024..1144914 - 891 WP_000971336.1 heme o synthase -
L6L55_RS06340 (1144926) 1144926..1145255 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
L6L55_RS06345 (1145255) 1145255..1145869 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
L6L55_RS06350 (1145859) 1145859..1147850 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
L6L55_RS06355 (1147872) 1147872..1148819 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T234565 WP_000227784.1 NZ_CP091700:c1143544-1143002 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T234565 NZ_LR130562:219591-219698 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT234565 WP_001297137.1 NZ_CP091700:c1143839-1143528 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT234565 NZ_LR130562:c219542-219479 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References