Detailed information of TA system TA234562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 664964..665540
• Replicon: chromosome
• Accession: NZ_CP091700
• Organism: Escherichia coli strain KFu019

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: L6L55_RS04045
• Protein ID: WP_001297643.1
• Coordinates: 664964..665251 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: L6L55_RS04050
• Protein ID: WP_000063156.1
• Coordinates: 665238..665540 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L55_RS04025 (661217)	661217..662497	-	1281	WP_001037382.1	DUF445 domain-containing protein	-
L6L55_RS04030 (662538)	662538..663770	-	1233	WP_214142381.1	multidrug efflux MFS transporter MdtM	-
L6L55_RS04035 (663916)	663916..664011	-	96	Protein_604	dienelactone hydrolase family protein	-
L6L55_RS04040 (664068)	664068..664765	+	698	Protein_605	IS1 family transposase	-
L6L55_RS04045 (664964)	664964..665251	+	288	WP_001297643.1	BrnT family toxin	Toxin
L6L55_RS04050 (665238)	665238..665540	+	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
L6L55_RS04055 (665574)	665574..666530	-	957	WP_001297640.1	GTPase	-
L6L55_RS04060 (666541)	666541..666744	-	204	WP_000467859.1	YbdD/YjiX family protein	-
L6L55_RS04065 (666794)	666794..668944	-	2151	WP_214142375.1	carbon starvation CstA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	664556..664765	209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T234562 WP_001297643.1 NZ_CP091700:664964-665251 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T234562 NZ_LR130558:8516-8770 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT234562 WP_000063156.1 NZ_CP091700:665238-665540 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT234562 NZ_LR130558:8767-9009 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Structures

Toxin

• AlphaFold DB: A0A829GEV9

Antitoxin

• AlphaFold DB: A0A829G8Z0