Detailed information of TA system TA023456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1544009..1544683
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7JBN6
• Locus tag: AFE_RS08255
• Protein ID: WP_009564531.1
• Coordinates: 1544264..1544683 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7JBN5
• Locus tag: AFE_RS08250
• Protein ID: WP_012536752.1
• Coordinates: 1544009..1544263 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS08235	1539684..1540688	-	1005	WP_012536749.1	glycosyltransferase family 2 protein	-
AFE_RS08240	1540827..1542287	+	1461	WP_012536750.1	IMP dehydrogenase	-
AFE_RS08245	1542291..1543835	+	1545	WP_012536751.1	glutamine-hydrolyzing GMP synthase	-
AFE_RS08250	1544009..1544263	+	255	WP_012536752.1	plasmid stabilization protein	Antitoxin
AFE_RS08255	1544264..1544683	+	420	WP_009564531.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFE_RS08260	1544701..1544913	+	213	WP_009564530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AFE_RS15680	1544913..1545068	+	156	WP_012536753.1	hypothetical protein	-
AFE_RS08265	1545748..1547367	+	1620	WP_012536754.1	APC family permease	-
AFE_RS08270	1547458..1548657	+	1200	WP_012536755.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14968.11 Da
• Isoelectric Point: 4.7051

>T23456 WP_009564531.1 NC_011761:1544264-1544683 [Acidithiobacillus ferrooxidans ATCC 23270]
MIVLDTNVVSEAMKPEPDAAVRAWLNEQAAETLYLSSVTLAELLFGIRALPAGKCKNMLDRALTELLELFKDRVLPFDTD
AARHYADLAVTAKNGGRGFPTPDGYIAAVAASRGFIVASRDTSPYEAAGVTVINPWKDA

Nucleotide

• Length: 420 bp

>T23456 NC_011761:1544264-1544683 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGATCGTCCTGGATACCAACGTCGTTTCCGAGGCGATGAAGCCGGAGCCAGACGCGGCCGTGAGGGCTTGGCTGAACGA
ACAGGCGGCGGAAACCTTGTATCTGTCGAGCGTCACGCTGGCCGAGTTGCTGTTCGGCATCCGGGCGCTGCCGGCGGGCA
AGTGCAAGAACATGCTCGACCGCGCCCTGACCGAACTGCTGGAGCTGTTCAAGGATCGGGTGCTGCCCTTCGATACGGAT
GCAGCGCGCCACTATGCCGACCTGGCCGTGACGGCCAAGAACGGCGGGCGCGGCTTCCCGACGCCAGACGGCTACATCGC
GGCTGTTGCCGCATCGCGGGGCTTCATCGTGGCATCACGCGACACGTCGCCCTATGAAGCGGCCGGCGTGACCGTCATCA
ATCCCTGGAAGGATGCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9225.67 Da
• Isoelectric Point: 10.7239

>AT23456 WP_012536752.1 NC_011761:1544009-1544263 [Acidithiobacillus ferrooxidans ATCC 23270]
MAILTVRNVPDDVHRALRVRAARHGRSTEAEVREILAAAVKPSSRVCMGEALAAIGRKIGLTNEDFAVFESVRDKTPAKP
LRFD

Nucleotide

• Length: 255 bp

>AT23456 NC_011761:1544009-1544263 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGCAATCCTGACTGTAAGAAATGTGCCGGATGACGTGCATCGCGCTTTGCGCGTGCGGGCGGCCCGGCACGGTCGCAG
CACGGAAGCGGAGGTGCGCGAGATTCTGGCGGCTGCGGTCAAACCTTCGAGCCGCGTGTGCATGGGGGAAGCCCTGGCCG
CCATCGGCCGCAAGATCGGTCTGACCAATGAAGATTTCGCCGTTTTCGAGAGTGTGCGCGACAAGACGCCGGCCAAACCG
TTGCGCTTCGACTGA

Structures

Toxin

• AlphaFold DB: B7JBN6

Antitoxin

• AlphaFold DB: B7JBN5