Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 20354..20997 Replicon plasmid pKMi006_1
Accession NZ_CP091692
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag L6L65_RS23720 Protein ID WP_001034044.1
Coordinates 20581..20997 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag L6L65_RS23715 Protein ID WP_001261286.1
Coordinates 20354..20584 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS23690 (16760) 16760..16999 + 240 Protein_16 TraX family protein -
L6L65_RS23700 (18394) 18394..19200 - 807 WP_021517370.1 site-specific integrase -
L6L65_RS23705 (19201) 19201..19506 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
L6L65_RS23710 (19508) 19508..19726 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
L6L65_RS23715 (20354) 20354..20584 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6L65_RS23720 (20581) 20581..20997 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
L6L65_RS23725 (21072) 21072..22637 + 1566 WP_021517369.1 AAA family ATPase -
L6L65_RS23730 (22622) 22622..23644 + 1023 WP_000361402.1 helicase UvrD -
L6L65_RS23735 (24341) 24341..24342 - 2 WP_001743396.1 IS1-like element IS1A family transposase -
L6L65_RS23740 (24950) 24950..25864 + 915 WP_000949005.1 iron/manganese ABC transporter substrate-binding protein SitA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B iucA / iucB / iucC / iucD / iutA / afaF-III / draA / draB / afaC-I / draD / draP 1..154958 154958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T234546 WP_001034044.1 NZ_CP091692:20581-20997 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T234546 NZ_LR130555:3779780-3779998 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT234546 WP_001261286.1 NZ_CP091692:20354-20584 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT234546 NZ_LR130555:3779380-3779754 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References