Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 19201..19726 Replicon plasmid pKMi006_1
Accession NZ_CP091692
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag L6L65_RS23705 Protein ID WP_001159871.1
Coordinates 19201..19506 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag L6L65_RS23710 Protein ID WP_000813630.1
Coordinates 19508..19726 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS23690 (16760) 16760..16999 + 240 Protein_16 TraX family protein -
L6L65_RS23700 (18394) 18394..19200 - 807 WP_021517370.1 site-specific integrase -
L6L65_RS23705 (19201) 19201..19506 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
L6L65_RS23710 (19508) 19508..19726 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L6L65_RS23715 (20354) 20354..20584 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
L6L65_RS23720 (20581) 20581..20997 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
L6L65_RS23725 (21072) 21072..22637 + 1566 WP_021517369.1 AAA family ATPase -
L6L65_RS23730 (22622) 22622..23644 + 1023 WP_000361402.1 helicase UvrD -
L6L65_RS23735 (24341) 24341..24342 - 2 WP_001743396.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / dfrA12 / aadA2 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1B iucA / iucB / iucC / iucD / iutA / afaF-III / draA / draB / afaC-I / draD / draP 1..154958 154958


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T234545 WP_001159871.1 NZ_CP091692:c19506-19201 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T234545 NZ_LR130555:c3752198-3751896 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT234545 WP_000813630.1 NZ_CP091692:c19726-19508 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT234545 NZ_LR130555:c3751860-3751519 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References