Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4561035..4561689 Replicon chromosome
Accession NZ_CP091691
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L6L65_RS22180 Protein ID WP_000244777.1
Coordinates 4561282..4561689 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L65_RS22175 Protein ID WP_000354046.1
Coordinates 4561035..4561301 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS22150 (4556204) 4556204..4556947 + 744 WP_000951964.1 SDR family oxidoreductase -
L6L65_RS22155 (4557004) 4557004..4558437 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
L6L65_RS22160 (4558482) 4558482..4558793 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
L6L65_RS22165 (4558957) 4558957..4559616 + 660 WP_000250274.1 hemolysin III family protein -
L6L65_RS22170 (4559812) 4559812..4560792 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L6L65_RS22175 (4561035) 4561035..4561301 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L65_RS22180 (4561282) 4561282..4561689 + 408 WP_000244777.1 protein YgfX Toxin
L6L65_RS22185 (4561729) 4561729..4562250 - 522 WP_001055874.1 flavodoxin FldB -
L6L65_RS22190 (4562362) 4562362..4563258 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L65_RS22195 (4563283) 4563283..4563993 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L65_RS22200 (4563999) 4563999..4565732 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234543 WP_000244777.1 NZ_CP091691:4561282-4561689 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234543 NZ_LR130555:3050084-3050578 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234543 WP_000354046.1 NZ_CP091691:4561035-4561301 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234543 NZ_LR130555:3049794-3050087 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References