Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4417727..4418562 Replicon chromosome
Accession NZ_CP091691
Organism Escherichia coli strain KMi006

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J1YPH7
Locus tag L6L65_RS21420 Protein ID WP_029701676.1
Coordinates 4417727..4418104 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K5N986
Locus tag L6L65_RS21425 Protein ID WP_021553056.1
Coordinates 4418194..4418562 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L65_RS21385 (4412808) 4412808..4413407 + 600 WP_001255034.1 type II secretion system minor pseudopilin GspJ -
L6L65_RS21390 (4413410) 4413410..4414387 + 978 WP_000633209.1 type II secretion system minor pseudopilin GspK -
L6L65_RS21395 (4414384) 4414384..4415562 + 1179 WP_000094991.1 type II secretion system protein GspL -
L6L65_RS21400 (4415564) 4415564..4416100 + 537 WP_000942785.1 GspM family type II secretion system protein YghD -
L6L65_RS21405 (4416381) 4416381..4417223 - 843 WP_022645115.1 DUF4942 domain-containing protein -
L6L65_RS21410 (4417308) 4417308..4417502 - 195 WP_024181941.1 DUF957 domain-containing protein -
L6L65_RS21415 (4417581) 4417581..4417730 - 150 Protein_4198 DUF5983 family protein -
L6L65_RS21420 (4417727) 4417727..4418104 - 378 WP_029701676.1 TA system toxin CbtA family protein Toxin
L6L65_RS21425 (4418194) 4418194..4418562 - 369 WP_021553056.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
L6L65_RS21430 (4418725) 4418725..4418946 - 222 WP_000692345.1 DUF987 domain-containing protein -
L6L65_RS21435 (4419011) 4419011..4419487 - 477 WP_240723978.1 RadC family protein -
L6L65_RS21440 (4419503) 4419503..4419982 - 480 WP_001564060.1 antirestriction protein -
L6L65_RS21445 (4420248) 4420248..4421066 - 819 WP_001175165.1 DUF932 domain-containing protein -
L6L65_RS21450 (4421156) 4421156..4421389 - 234 WP_001278293.1 DUF905 family protein -
L6L65_RS21455 (4421395) 4421395..4422072 - 678 WP_001097302.1 hypothetical protein -
L6L65_RS21460 (4422220) 4422220..4422900 - 681 WP_029702103.1 WYL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 4416254..4491686 75432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14066.04 Da        Isoelectric Point: 7.8045

>T234542 WP_029701676.1 NZ_CP091691:c4418104-4417727 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T234542 NZ_LR130555:2890668-2890775 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13615.39 Da        Isoelectric Point: 7.0264

>AT234542 WP_021553056.1 NZ_CP091691:c4418562-4418194 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT234542 NZ_LR130555:c2890618-2890561 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1YPH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K5N986

References