Detailed information of TA system TA023454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1390753..1391790
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: AFE_RS07490
• Protein ID: WP_009566509.1
• Coordinates: 1390753..1391325 (-)
• Length: 191 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B7JAV4
• Locus tag: AFE_RS07495
• Protein ID: WP_009566508.1
• Coordinates: 1391326..1391790 (-)
• Length: 155 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS07470	1386247..1387242	-	996	WP_012607128.1	efflux RND transporter periplasmic adaptor subunit	-
AFE_RS07475	1387850..1389091	-	1242	Protein_1441	IS3 family transposase	-
AFE_RS16595	1389601..1390200	-	600	Protein_1442	transposase	-
AFE_RS07490	1390753..1391325	-	573	WP_009566509.1	PIN domain-containing protein	Toxin
AFE_RS07495	1391326..1391790	-	465	WP_009566508.1	helix-turn-helix domain-containing protein	Antitoxin
AFE_RS15640	1391808..1392086	-	279	WP_009566507.1	TIR domain-containing protein	-
AFE_RS07500	1392226..1396575	+	4350	WP_012607132.1	DUF3560 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1368452..1392086	23634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-130)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 21854.10 Da
• Isoelectric Point: 6.2929

>T23454 WP_009566509.1 NC_011761:c1391325-1390753 [Acidithiobacillus ferrooxidans ATCC 23270]
MSSNFIVVYDACVLYPAPLRDLLMRLALTDLYRARWTDMIHDEWTRNVLKQRPDLKAEDLERTRLLMNDHVRDSLVTGFE
HLIPSVELPDADDRHVVAAAIHGGASLIVTFNLKDFPPERLKPYNLTAQHPDDFIFDLLDLHAARVCEAAANHRRSLKYP
PKTVDEYLDTLLKQGLTQTVGQLREWKVAI

Nucleotide

• Length: 573 bp

>T23454 NC_011761:c1391325-1390753 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGAGTTCGAACTTCATCGTCGTCTACGACGCGTGCGTGCTCTACCCGGCACCGTTGCGCGACCTGCTGATGCGCCTGGC
GCTGACCGATCTGTACCGGGCGCGTTGGACGGACATGATCCACGACGAGTGGACGCGTAACGTTCTGAAGCAGCGGCCAG
ATCTGAAGGCCGAAGACCTGGAGCGAACGCGGTTGTTGATGAATGACCATGTCCGCGACAGCCTGGTCACCGGCTTCGAG
CATCTGATCCCGTCCGTCGAGTTGCCGGACGCCGATGACCGGCATGTGGTGGCTGCCGCTATTCACGGTGGTGCCAGCCT
GATCGTGACGTTCAACCTGAAGGATTTTCCGCCGGAACGGCTCAAACCCTACAACCTGACCGCCCAGCATCCCGACGACT
TCATCTTCGATCTACTGGATCTGCACGCAGCCCGAGTGTGCGAGGCGGCTGCCAACCATCGCCGATCGCTGAAGTACCCA
CCCAAGACAGTTGACGAGTACCTAGACACGCTGCTCAAGCAGGGACTGACACAGACCGTCGGCCAGTTGCGGGAGTGGAA
GGTAGCGATCTGA

Antitoxin

Protein

• Length: 155 a.a.
• Molecular weight: 17042.37 Da
• Isoelectric Point: 6.3391

>AT23454 WP_009566508.1 NC_011761:c1391790-1391326 [Acidithiobacillus ferrooxidans ATCC 23270]
MTTRSPTHSLPTAEEVAIARESGRALSAFLQTRAETQQIEIFNDKGASHSVRVPVSALRLLVDVLTEIGEGNAVSIIPIH
AELTTQDAADVLNVSRPFLVQLLERGEIPFHKIGTHRRVRYQDVIAYKERIDAERSKALDALAEQAQALKMGYE

Nucleotide

• Length: 465 bp

>AT23454 NC_011761:c1391790-1391326 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGACCACACGCTCTCCCACCCATTCGTTGCCGACGGCCGAAGAAGTTGCCATCGCCCGTGAGAGCGGCCGGGCGCTGTC
CGCCTTCCTTCAGACCCGCGCGGAAACCCAGCAGATTGAGATATTCAACGACAAGGGCGCGTCTCATTCGGTCCGCGTCC
CAGTGTCGGCGCTGCGCCTTCTCGTGGACGTACTGACCGAAATCGGTGAGGGCAACGCGGTCAGTATCATCCCGATCCAC
GCCGAACTGACAACACAAGACGCAGCGGACGTACTCAACGTCTCGCGACCGTTCCTCGTCCAGTTGCTGGAGCGCGGTGA
GATCCCATTCCACAAGATCGGAACTCATCGTCGTGTCCGTTACCAGGACGTGATCGCGTACAAGGAGCGGATCGACGCTG
AGCGCAGCAAGGCCCTTGATGCACTGGCCGAGCAAGCCCAGGCGCTCAAGATGGGGTACGAATGA

Structures

Antitoxin

• AlphaFold DB: B7JAV4