Detailed information of TA system TA023453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 1371201..1371769
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: B7JAF4
• Locus tag: AFE_RS07375
• Protein ID: WP_009565773.1
• Coordinates: 1371201..1371575 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AFE_RS15630
• Protein ID: WP_012607109.1
• Coordinates: 1371572..1371769 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS07365	1368452..1369651	-	1200	WP_012607107.1	restriction endonuclease subunit S	-
AFE_RS07370	1369648..1371102	-	1455	WP_012607108.1	type I restriction-modification system subunit M	-
AFE_RS07375	1371201..1371575	-	375	WP_009565773.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFE_RS15630	1371572..1371769	-	198	WP_012607109.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AFE_RS16460	1372085..1372246	-	162	WP_012536621.1	hypothetical protein	-
AFE_RS07380	1372599..1373273	+	675	WP_012607112.1	hypothetical protein	-
AFE_RS07385	1373270..1373593	+	324	WP_012607113.1	integration host factor subunit alpha	-
AFE_RS07390	1373660..1373935	+	276	WP_012536624.1	HU family DNA-binding protein	-
AFE_RS07395	1373947..1374300	+	354	WP_009560827.1	hypothetical protein	-
AFE_RS15635	1374309..1374530	+	222	WP_009560828.1	HU family DNA-binding protein	-
AFE_RS07400	1375133..1375267	-	135	Protein_1426	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
AFE_RS07405	1375254..1375523	-	270	WP_012536625.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
AFE_RS07410	1375548..1376444	-	897	WP_009560830.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1368452..1392086	23634

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13625.67 Da
• Isoelectric Point: 6.3157

>T23453 WP_009565773.1 NC_011761:c1371575-1371201 [Acidithiobacillus ferrooxidans ATCC 23270]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSGLPRCTATSPEETLFFIGRHGLMGR
GIGYIDASILASAMLVQANLWSRDKRLMALATELGCAYSPTSSE

Nucleotide

• Length: 375 bp

>T23453 NC_011761:c1371575-1371201 [Acidithiobacillus ferrooxidans ATCC 23270]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTTCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAGCAGACCTTATATCTGC
TCTCAGGCTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTTATTGGGCGTCACGGCCTGATGGGACGC
GGTATAGGCTATATTGACGCGAGTATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAAACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCGCCGACGTCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7254.32 Da
• Isoelectric Point: 9.4896

>AT23453 WP_012607109.1 NC_011761:c1371769-1371572 [Acidithiobacillus ferrooxidans ATCC 23270]
MRTTINLDDELLAQAKTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNTPRRREPAA

Nucleotide

• Length: 198 bp

>AT23453 NC_011761:c1371769-1371572 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAAAACGCTCTGTGGCGTAGAAGACCGTGGTGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCCTGGGTGGAACAGAACCGCAGT
TACAGAACACTCCTCGCAGACGAGAGCCTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: B7JAF4