Detailed information of TA system TA234522

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 665752..666587
• Replicon: chromosome
• Accession: NZ_CP091691
• Organism: Escherichia coli strain KMi006

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7X1VUT4
• Locus tag: L6L65_RS03125
• Protein ID: WP_025693544.1
• Coordinates: 665752..666129 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: L6L65_RS03130
• Protein ID: WP_001360327.1
• Coordinates: 666219..666587 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L65_RS03090 (661272)	661272..661745	+	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-
L6L65_RS03095 (661943)	661943..663001	+	1059	WP_001200895.1	FUSC family protein	-
L6L65_RS03100 (663173)	663173..663502	+	330	WP_000450409.1	DUF496 family protein	-
L6L65_RS03105 (663603)	663603..663968	-	366	WP_001280454.1	EutP/PduV family microcompartment system protein	-
L6L65_RS03110 (664239)	664239..664586	-	348	WP_001333417.1	IS110 family transposase	-
L6L65_RS03115 (665426)	665426..665506	-	81	Protein_613	hypothetical protein	-
L6L65_RS03120 (665606)	665606..665755	-	150	Protein_614	DUF5983 family protein	-
L6L65_RS03125 (665752)	665752..666129	-	378	WP_025693544.1	TA system toxin CbtA family protein	Toxin
L6L65_RS03130 (666219)	666219..666587	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L6L65_RS03135 (666750)	666750..666971	-	222	WP_000692286.1	DUF987 domain-containing protein	-
L6L65_RS03140 (667034)	667034..667510	-	477	WP_001186774.1	RadC family protein	-
L6L65_RS03145 (667526)	667526..667999	-	474	WP_000855059.1	antirestriction protein	-
L6L65_RS03150 (668104)	668104..669643	-	1540	Protein_620	IS66-like element ISEc22 family transposase	-
L6L65_RS03155 (669692)	669692..670039	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-
L6L65_RS03160 (670036)	670036..670440	-	405	WP_000839179.1	transposase	-
L6L65_RS03165 (670469)	670469..670804	-	336	WP_240724006.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14039.01 Da
• Isoelectric Point: 7.2919

>T234522 WP_025693544.1 NZ_CP091691:c666129-665752 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T234522 NZ_LR130552:3765594-3765872 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT234522 WP_001360327.1 NZ_CP091691:c666587-666219 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT234522 NZ_LR130552:3765331-3765591 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7X1VUT4

Antitoxin

• AlphaFold DB: A0A7U9IW59