Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 79363..80006 Replicon plasmid pKKo003_2
Accession NZ_CP091685
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag L6L54_RS25210 Protein ID WP_001034044.1
Coordinates 79590..80006 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag L6L54_RS25205 Protein ID WP_001261286.1
Coordinates 79363..79593 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS25190 (L6L54_25190) 74500..74730 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
L6L54_RS25195 (L6L54_25195) 74727..75143 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
L6L54_RS25200 (L6L54_25200) 75188..78982 - 3795 WP_001144732.1 hypothetical protein -
L6L54_RS25205 (L6L54_25205) 79363..79593 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6L54_RS25210 (L6L54_25210) 79590..80006 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
L6L54_RS25215 (L6L54_25215) 80081..81646 + 1566 WP_001128474.1 AAA family ATPase -
L6L54_RS25220 (L6L54_25220) 81631..82653 + 1023 WP_000361402.1 helicase UvrD -
L6L54_RS25230 (L6L54_25230) 83907..84604 + 698 WP_223155668.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B senB 1..86532 86532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T234520 WP_001034044.1 NZ_CP091685:79590-80006 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T234520 NZ_LR130552:c3489624-3489322 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCTCGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT234520 WP_001261286.1 NZ_CP091685:79363-79593 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT234520 NZ_LR130552:c3489286-3488945 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References