Detailed information of TA system TA023452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1225026..1225789
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: B7J9L5
• Locus tag: AFE_RS06630
• Protein ID: WP_009565062.1
• Coordinates: 1225026..1225520 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: B7J9L6
• Locus tag: AFE_RS06635
• Protein ID: WP_012536505.1
• Coordinates: 1225517..1225789 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS06605	1220803..1221573	+	771	WP_012536502.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AFE_RS06610	1221566..1222408	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
AFE_RS06615	1223126..1223632	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	-
AFE_RS06620	1223629..1223958	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	-
AFE_RS16455	1224245..1224388	+	144	WP_009569579.1	hypothetical protein	-
AFE_RS06625	1224415..1224681	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
AFE_RS06630	1225026..1225520	-	495	WP_009565062.1	GNAT family N-acetyltransferase	Toxin
AFE_RS06635	1225517..1225789	-	273	WP_012536505.1	DUF1778 domain-containing protein	Antitoxin
AFE_RS06640	1225870..1226082	-	213	WP_009562915.1	hypothetical protein	-
AFE_RS06645	1226250..1226466	-	217	Protein_1270	hypothetical protein	-
AFE_RS06650	1226866..1227687	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
AFE_RS06655	1227928..1228173	+	246	WP_009568482.1	DUF2934 domain-containing protein	-
AFE_RS06660	1228368..1229504	+	1137	WP_012536507.1	glycosyltransferase family 1 protein	-
AFE_RS06665	1229538..1230752	+	1215	WP_012536508.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-87)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18013.83 Da
• Isoelectric Point: 10.4415

>T23452 WP_009565062.1 NC_011761:c1225520-1225026 [Acidithiobacillus ferrooxidans ATCC 23270]
MSLKLSAPQPLAAVHLLGDFESGEPALDEWLKRRAMNNQLTGASRTFVVVDEENRVRGFYAMAAGAVAHQVATSSVRRNM
PDPVPVMVLGRLAVDSRAQGIKLGAAMLQDAVKRAITVSQNTGVRALLVHALHERAKQFYEHYGFQESPQHPMTLMLRLN
SIKA

Nucleotide

• Length: 495 bp

>T23452 NC_011761:c1225520-1225026 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGAGTTTGAAGCTATCCGCGCCGCAGCCACTTGCGGCCGTGCACCTGTTGGGCGACTTCGAGAGCGGTGAGCCTGCACT
GGATGAGTGGTTAAAACGCCGGGCGATGAACAACCAGCTCACCGGGGCCAGTCGAACCTTTGTGGTCGTTGATGAAGAAA
ATCGGGTGCGCGGCTTCTACGCCATGGCGGCTGGCGCAGTCGCACATCAAGTGGCGACTAGCTCGGTTCGTCGCAATATG
CCCGATCCGGTTCCCGTCATGGTGCTCGGTAGGTTGGCGGTGGATAGTCGAGCGCAAGGCATCAAGCTGGGGGCGGCCAT
GCTGCAGGACGCGGTGAAACGTGCCATCACCGTATCGCAGAACACGGGGGTTCGTGCACTGCTGGTTCATGCGCTCCACG
AACGCGCCAAACAGTTCTATGAGCACTACGGCTTTCAGGAGTCACCACAACATCCGATGACGCTGATGTTGCGCCTGAAT
AGCATCAAGGCTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10071.52 Da
• Isoelectric Point: 5.0914

>AT23452 WP_012536505.1 NC_011761:c1225789-1225517 [Acidithiobacillus ferrooxidans ATCC 23270]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVLLDQVFFGLDAEKFRQFTAMLDAPPRGNEGLERLMA
VKAPWESNQA

Nucleotide

• Length: 273 bp

>AT23452 NC_011761:c1225789-1225517 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGCGTGCCGCTGCCATCAATTTACGAGCCTTGCCGGAGCAGCGTGATCTCATTGATCACGCCGCCAACCTGTTAGGCAA
AAACCGTTCGGACTTCATGCTGGAAGCCGCCTGCGAGCGTGCGCAATCGGTACTGCTCGATCAGGTGTTTTTTGGGCTGG
ATGCGGAAAAGTTCCGGCAGTTCACCGCCATGCTCGACGCACCGCCTCGTGGCAACGAAGGTCTTGAGCGTCTGATGGCA
GTCAAGGCGCCATGGGAAAGCAATCAAGCATGA

Structures

Toxin

• AlphaFold DB: B7J9L5

Antitoxin

• AlphaFold DB: B7J9L6