234519

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 74500..75143 Replicon plasmid pKKo003_2
Accession NZ_CP091685
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag L6L54_RS25195 Protein ID WP_001034046.1
Coordinates 74727..75143 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag L6L54_RS25190 Protein ID WP_001261278.1
Coordinates 74500..74730 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS25160 (L6L54_25160) 70011..70316 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
L6L54_RS25165 (L6L54_25165) 70318..70536 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
L6L54_RS25170 (L6L54_25170) 71103..71615 + 513 WP_000151784.1 hypothetical protein -
L6L54_RS25175 (L6L54_25175) 71649..72782 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
L6L54_RS25180 (L6L54_25180) 72949..73722 - 774 WP_000905949.1 hypothetical protein -
L6L54_RS25185 (L6L54_25185) 73735..74235 - 501 WP_001773886.1 HEPN family nuclease -
L6L54_RS25190 (L6L54_25190) 74500..74730 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6L54_RS25195 (L6L54_25195) 74727..75143 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
L6L54_RS25200 (L6L54_25200) 75188..78982 - 3795 WP_001144732.1 hypothetical protein -
L6L54_RS25205 (L6L54_25205) 79363..79593 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
L6L54_RS25210 (L6L54_25210) 79590..80006 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B senB 1..86532 86532


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T234519 WP_001034046.1 NZ_CP091685:74727-75143 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T234519 NZ_LR130552:2701760-2701867 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT234519 WP_001261278.1 NZ_CP091685:74500-74730 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT234519 NZ_LR130552:c2701710-2701647 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References