Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 1778..2337 Replicon plasmid pKKo003_1
Accession NZ_CP091684
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name relE Uniprot ID U9YB49
Locus tag L6L54_RS24170 Protein ID WP_000421259.1
Coordinates 2062..2337 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID U9Y625
Locus tag L6L54_RS24165 Protein ID WP_001178087.1
Coordinates 1778..2062 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS24160 (L6L54_24160) 1..879 + 879 WP_000729439.1 incFII family plasmid replication initiator RepA -
L6L54_RS24165 (L6L54_24165) 1778..2062 + 285 WP_001178087.1 ribbon-helix-helix domain-containing protein Antitoxin
L6L54_RS24170 (L6L54_24170) 2062..2337 + 276 WP_000421259.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6L54_RS24175 (L6L54_24175) 2443..2640 + 198 Protein_3 hypothetical protein -
L6L54_RS24180 (L6L54_24180) 2909..3175 - 267 WP_000627832.1 hypothetical protein -
L6L54_RS24185 (L6L54_24185) 3175..3393 - 219 Protein_5 hypothetical protein -
L6L54_RS24190 (L6L54_24190) 3421..4023 - 603 WP_000521606.1 ProQ/FINO family protein -
L6L54_RS24195 (L6L54_24195) 4199..5251 - 1053 WP_000655519.1 hypothetical protein -
L6L54_RS24200 (L6L54_24200) 5464..6054 - 591 WP_001401589.1 hypothetical protein -
L6L54_RS24205 (L6L54_24205) 6054..6308 - 255 WP_000343106.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid cmlA1 / qacE / blaCTX-M-14 - 1..100833 100833


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10736.45 Da        Isoelectric Point: 9.9324

>T234517 WP_000421259.1 NZ_CP091684:2062-2337 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T234517 NZ_LR130552:2700689-2700796 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10488.92 Da        Isoelectric Point: 5.6558

>AT234517 WP_001178087.1 NZ_CP091684:1778-2062 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT234517 NZ_LR130552:c2700642-2700576 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YB49

Antitoxin

Source ID Structure
AlphaFold DB U9Y625

References