Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3771638..3772292 Replicon chromosome
Accession NZ_CP091682
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag L6L54_RS18395 Protein ID WP_000244781.1
Coordinates 3771638..3772045 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L54_RS18400 Protein ID WP_000354046.1
Coordinates 3772026..3772292 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS18375 (3767595) 3767595..3769328 - 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -
L6L54_RS18380 (3769334) 3769334..3770044 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L54_RS18385 (3770069) 3770069..3770965 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L54_RS18390 (3771077) 3771077..3771598 + 522 WP_001055874.1 flavodoxin FldB -
L6L54_RS18395 (3771638) 3771638..3772045 - 408 WP_000244781.1 protein YgfX Toxin
L6L54_RS18400 (3772026) 3772026..3772292 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L54_RS18405 (3772535) 3772535..3773515 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
L6L54_RS18410 (3773592) 3773592..3774251 - 660 WP_000250274.1 hemolysin III family protein -
L6L54_RS18415 (3774415) 3774415..3774726 - 312 WP_001182959.1 N(4)-acetylcytidine aminohydrolase -
L6L54_RS18420 (3774771) 3774771..3776204 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T234512 WP_000244781.1 NZ_CP091682:c3772045-3771638 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234512 NZ_LR130552:1362524-1362922 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234512 WP_000354046.1 NZ_CP091682:c3772292-3772026 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234512 NZ_LR130552:1362297-1362524 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References