Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3615711..3616294 Replicon chromosome
Accession NZ_CP091682
Organism Escherichia coli strain KKo003

Toxin (Protein)


Gene name mazF Uniprot ID S1PVD8
Locus tag L6L54_RS17770 Protein ID WP_000254749.1
Coordinates 3615711..3616046 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag L6L54_RS17775 Protein ID WP_000581937.1
Coordinates 3616046..3616294 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L54_RS17755 (3611597) 3611597..3612895 - 1299 WP_000036723.1 phosphopyruvate hydratase -
L6L54_RS17760 (3612983) 3612983..3614620 - 1638 WP_001774030.1 CTP synthase (glutamine hydrolyzing) -
L6L54_RS17765 (3614848) 3614848..3615639 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
L6L54_RS17770 (3615711) 3615711..3616046 - 336 WP_000254749.1 endoribonuclease MazF Toxin
L6L54_RS17775 (3616046) 3616046..3616294 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
L6L54_RS17780 (3616372) 3616372..3618606 - 2235 WP_000226797.1 GTP diphosphokinase -
L6L54_RS17785 (3618654) 3618654..3619955 - 1302 WP_000046817.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12137.06 Da        Isoelectric Point: 8.7218

>T234511 WP_000254749.1 NZ_CP091682:c3616046-3615711 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKR
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T234511 NZ_LR130552:1063666-1064001 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT234511 WP_000581937.1 NZ_CP091682:c3616294-3616046 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT234511 NZ_LR130552:1063418-1063666 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PVD8

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References