Detailed information of TA system TA023449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 991885..992465
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B7J847
• Locus tag: AFE_RS05160
• Protein ID: WP_012606809.1
• Coordinates: 992124..992465 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: B7J846
• Locus tag: AFE_RS05155
• Protein ID: WP_012606808.1
• Coordinates: 991885..992124 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS15265	987174..987455	+	282	WP_049759419.1	hypothetical protein	-
AFE_RS05145	987452..988702	+	1251	WP_012606806.1	TraU family protein	-
AFE_RS05150	988768..991827	+	3060	WP_012606807.1	conjugal transfer protein TraN	-
AFE_RS05155	991885..992124	+	240	WP_012606808.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AFE_RS05160	992124..992465	+	342	WP_012606809.1	endoribonuclease MazF	Toxin
AFE_RS05165	992532..993596	+	1065	WP_049759493.1	hypothetical protein	-
AFE_RS05170	993877..994122	+	246	WP_041645457.1	hypothetical protein	-
AFE_RS05175	995241..996518	+	1278	WP_012606814.1	hypothetical protein	-
AFE_RS05180	996622..997212	+	591	WP_012606815.1	IS607 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-103)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12332.25 Da
• Isoelectric Point: 7.8030

>T23449 WP_012606809.1 NC_011761:992124-992465 [Acidithiobacillus ferrooxidans ATCC 23270]
MGAAYVPEAGDVVWLHFDPQAGHEQKGHRPALVLTSASYHAKTGLMVCCPMTTQAKGYPFEVRIAGDIPGVVLADQVKSL
DWEERQVRFKGRVTALELDEARAKACVLIGRKA

Nucleotide

• Length: 342 bp

>T23449 NC_011761:992124-992465 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGGCGCTGCCTATGTTCCGGAAGCTGGCGATGTCGTCTGGCTACATTTTGACCCGCAGGCTGGTCACGAACAAAAGGG
CCACCGGCCCGCTTTGGTGCTGACATCCGCGAGTTACCATGCAAAAACGGGACTGATGGTTTGCTGTCCGATGACCACTC
AGGCCAAGGGATATCCGTTTGAAGTGCGCATCGCAGGAGATATCCCCGGCGTGGTGCTTGCGGACCAGGTGAAAAGCCTG
GATTGGGAGGAGCGCCAAGTCAGATTCAAAGGGCGGGTGACGGCGCTGGAACTGGATGAGGCAAGGGCGAAGGCCTGCGT
GCTGATTGGCCGCAAGGCATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8729.13 Da
• Isoelectric Point: 4.7769

>AT23449 WP_012606808.1 NC_011761:991885-992124 [Acidithiobacillus ferrooxidans ATCC 23270]
MRVIVKKWGNSAAVRMPAAVMEAAQLHLDDTVDVREEGGRIIIETLRQKFDLEEMVAAITPENMHAAVDFGPAVGRELL

Nucleotide

• Length: 240 bp

>AT23449 NC_011761:991885-992124 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGCGGGTGATTGTGAAAAAGTGGGGGAATAGCGCTGCCGTACGCATGCCTGCCGCCGTCATGGAAGCGGCTCAATTGCA
CCTGGATGATACTGTGGATGTGCGGGAAGAGGGTGGGCGTATTATCATCGAAACACTTCGGCAAAAGTTCGATCTGGAGG
AGATGGTAGCGGCCATTACGCCGGAGAACATGCATGCTGCAGTCGATTTTGGGCCGGCAGTTGGCAGGGAACTGCTCTGA

Structures

Toxin

• AlphaFold DB: B7J847

Antitoxin

• AlphaFold DB: B7J846