Detailed information of TA system TA234487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 92198..92799
• Replicon: plasmid pKFu022_2
• Accession: NZ_CP091679
• Organism: Escherichia coli strain KFu022

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: L6L66_RS23950
• Protein ID: WP_097456291.1
• Coordinates: 92198..92578 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: L6L66_RS23955
• Protein ID: WP_001190712.1
• Coordinates: 92578..92799 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L66_RS23925 (L6L66_23925)	87348..88832	-	1485	WP_000124150.1	terminase	-
L6L66_RS23930 (L6L66_23930)	88832..90025	-	1194	WP_000219616.1	hypothetical protein	-
L6L66_RS23935 (L6L66_23935)	90107..90559	-	453	WP_001326849.1	late promoter-activating protein	-
L6L66_RS23940 (L6L66_23940)	90648..91691	-	1044	WP_171865275.1	DUF968 domain-containing protein	-
L6L66_RS23945 (L6L66_23945)	91719..91898	-	180	WP_001339207.1	hypothetical protein	-
L6L66_RS23950 (L6L66_23950)	92198..92578	-	381	WP_097456291.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
L6L66_RS23955 (L6L66_23955)	92578..92799	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L6L66_RS23960 (L6L66_23960)	92872..93261	-	390	WP_000506727.1	S24 family peptidase	-
L6L66_RS23965 (L6L66_23965)	93436..94017	+	582	WP_113968900.1	DNA-binding protein	-
L6L66_RS23970 (L6L66_23970)	94078..94602	-	525	WP_113968902.1	toprim domain-containing protein	-
L6L66_RS23975 (L6L66_23975)	94599..94904	-	306	WP_000988220.1	hypothetical protein	-
L6L66_RS23980 (L6L66_23980)	94904..95188	-	285	WP_001142392.1	hypothetical protein	-
L6L66_RS23985 (L6L66_23985)	95172..95822	-	651	WP_240752881.1	hypothetical protein	-
L6L66_RS23990 (L6L66_23990)	95804..96178	-	375	WP_032219055.1	hypothetical protein	-
L6L66_RS23995 (L6L66_23995)	96185..96478	-	294	WP_000269004.1	hypothetical protein	-
L6L66_RS24000 (L6L66_24000)	96657..96890	-	234	WP_000517421.1	hypothetical protein	-
L6L66_RS24005 (L6L66_24005)	96973..97140	-	168	WP_233042539.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98126	98126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13671.51 Da
• Isoelectric Point: 5.6513

>T234487 WP_097456291.1 NZ_CP091679:c92578-92198 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELVDLTVGAATGEIAVSSVAEKLRELYGSKT

Nucleotide

• Length: 381 bp

>T234487 NZ_LR130545:c4621548-4621375 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT234487 WP_001190712.1 NZ_CP091679:c92799-92578 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT234487 NZ_LR130545:c4621872-4621576 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6