Detailed information of TA system TA023448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 804909..805434
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: B7J6U7
• Locus tag: AFE_RS04175
• Protein ID: WP_009564272.1
• Coordinates: 805153..805434 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B7J6U6
• Locus tag: AFE_RS04170
• Protein ID: WP_009564270.1
• Coordinates: 804909..805163 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS04130	799956..800627	-	672	WP_012536411.1	LysR family transcriptional regulator	-
AFE_RS04135	800825..801269	+	445	Protein_817	SDR family oxidoreductase	-
AFE_RS04140	801438..802106	+	669	WP_009561118.1	cupin domain-containing protein	-
AFE_RS04145	802103..803266	+	1164	WP_012536412.1	FAD-dependent oxidoreductase	-
AFE_RS04150	803263..803352	+	90	Protein_820	short-chain dehydrogenase	-
AFE_RS04155	803324..803609	+	286	Protein_821	hypothetical protein	-
AFE_RS15220	803840..804175	+	336	WP_012536413.1	shufflon-specific DNA recombinase	-
AFE_RS04165	804362..804661	+	300	WP_009564269.1	YbjQ family protein	-
AFE_RS04170	804909..805163	+	255	WP_009564270.1	antitoxin	Antitoxin
AFE_RS04175	805153..805434	+	282	WP_009564272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AFE_RS04185	805977..807200	-	1224	WP_012606680.1	class I SAM-dependent rRNA methyltransferase	-
AFE_RS04190	807355..808407	+	1053	WP_012536417.1	S-methyl-5-thioribose-1-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10757.61 Da
• Isoelectric Point: 10.6855

>T23448 WP_009564272.1 NC_011761:805153-805434 [Acidithiobacillus ferrooxidans ATCC 23270]
MPYELTFHPSALKEWQALDHSVRAPFKKKLAERLVHPRVPSAQLHGHPDRYKIKLRSIGYRLIYQVMETEVQVLVLAVGK
REGSVAYHKAKGR

Nucleotide

• Length: 282 bp

>T23448 NC_011761:805153-805434 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGCCTTATGAGCTCACTTTCCATCCCTCCGCCCTCAAAGAATGGCAGGCCTTGGACCATAGCGTCCGTGCCCCATTCAA
AAAGAAACTGGCAGAACGCTTAGTGCACCCACGCGTCCCTTCCGCTCAACTGCATGGCCACCCGGATCGCTATAAGATCA
AACTCCGCAGCATAGGCTATCGCTTGATCTACCAAGTGATGGAAACGGAAGTCCAAGTGTTGGTCCTGGCGGTAGGCAAG
CGGGAAGGCTCTGTCGCCTATCACAAGGCCAAAGGACGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8832.16 Da
• Isoelectric Point: 4.6615

>AT23448 WP_009564270.1 NC_011761:804909-805163 [Acidithiobacillus ferrooxidans ATCC 23270]
MTHVILADVAASVSELKKNPMGTVAAGEGATVAILNRNEPAFYCVPAATYAALLEHLENLELNALAESRLKDGQTPIKVS
LDAL

Nucleotide

• Length: 255 bp

>AT23448 NC_011761:804909-805163 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGACCCACGTCATTCTTGCCGATGTTGCTGCCAGTGTTTCCGAACTCAAAAAGAACCCTATGGGAACCGTGGCCGCAGG
AGAAGGGGCTACTGTGGCCATTCTCAACCGCAACGAACCGGCTTTTTACTGTGTCCCAGCCGCTACCTATGCTGCGCTTC
TGGAACATCTGGAAAATTTGGAGCTGAACGCTTTGGCGGAAAGCCGTCTGAAGGATGGCCAAACCCCGATCAAGGTGTCG
CTGGATGCCTTATGA

Structures

Toxin

• AlphaFold DB: B7J6U7

Antitoxin

• AlphaFold DB: B7J6U6