Detailed information of TA system TA023447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 418549..419189
• Replicon: chromosome
• Accession: NC_011761
• Organism: Acidithiobacillus ferrooxidans ATCC 23270

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AFE_RS02260
• Protein ID: WP_009567232.1
• Coordinates: 418779..419189 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: B7J4M0
• Locus tag: AFE_RS02255
• Protein ID: WP_009567231.1
• Coordinates: 418549..418779 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AFE_RS02240	414427..415557	+	1131	WP_012536159.1	threonine synthase	-
AFE_RS02245	415557..416522	+	966	WP_009567244.1	hypothetical protein	-
AFE_RS02250	416523..418340	+	1818	WP_012536160.1	single-stranded DNA exonuclease	-
AFE_RS02255	418549..418779	+	231	WP_009567231.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AFE_RS02260	418779..419189	+	411	WP_009567232.1	type II toxin-antitoxin system VapC family toxin	Toxin
AFE_RS02265	419322..420420	+	1099	WP_012606567.1	peptide chain release factor 2	-
AFE_RS02270	420457..421947	+	1491	WP_009568984.1	lysine--tRNA ligase	-
AFE_RS02275	421944..423188	+	1245	WP_012536161.1	lipoprotein-releasing ABC transporter permease subunit	-
AFE_RS02280	423181..423876	+	696	WP_009566877.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-43)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15278.46 Da
• Isoelectric Point: 7.3848

>T23447 WP_009567232.1 NC_011761:418779-419189 [Acidithiobacillus ferrooxidans ATCC 23270]
MTLRYLLDTNICIYIAKHHPPAVRERFVQHGADDLAMSVITLGELCWGAEKSQARDKALASIQRLQERIQVAPLTPQVGI
HYGQIRAALESGGRPIGNNDLWIAAHARAEGWIVVTNNEREFRRVEGLQVENWVTA

Nucleotide

• Length: 411 bp

>T23447 NC_011761:418779-419189 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGACCTTGCGTTATCTGCTGGATACCAATATCTGCATTTATATTGCCAAACACCATCCGCCCGCCGTGCGGGAGCGTTT
TGTACAACATGGTGCCGATGATCTGGCGATGTCGGTAATCACCTTGGGTGAATTGTGTTGGGGTGCGGAAAAAAGTCAGG
CGCGTGACAAAGCGTTGGCCAGCATACAGCGCCTGCAAGAACGTATTCAGGTGGCCCCCCTCACCCCTCAGGTCGGCATC
CACTATGGCCAGATCCGCGCGGCTCTGGAAAGCGGCGGGCGGCCCATTGGCAATAATGATCTGTGGATCGCCGCACACGC
CCGCGCCGAAGGCTGGATCGTAGTTACCAACAATGAACGAGAGTTCCGACGGGTGGAAGGATTGCAAGTGGAAAACTGGG
TAACGGCATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8704.76 Da
• Isoelectric Point: 4.4217

>AT23447 WP_009567231.1 NC_011761:418549-418779 [Acidithiobacillus ferrooxidans ATCC 23270]
MAIARIFQSGNSQAVRLPKEFRFEVDQVEIFRRGNEIILREHPVDGTGVFDALADLPEDFMSDGRQDIPPQARENF

Nucleotide

• Length: 231 bp

>AT23447 NC_011761:418549-418779 [Acidithiobacillus ferrooxidans ATCC 23270]
ATGGCCATCGCACGCATCTTCCAATCAGGCAACAGTCAGGCCGTACGGTTGCCCAAAGAATTCCGCTTTGAGGTGGATCA
GGTGGAAATCTTCCGCCGCGGGAATGAGATTATTCTGCGGGAGCATCCAGTGGATGGTACAGGCGTTTTTGATGCCCTGG
CCGACCTTCCCGAAGACTTCATGTCCGACGGACGCCAGGATATTCCACCCCAGGCAAGGGAAAACTTTTGA

Structures

Antitoxin

• AlphaFold DB: B7J4M0