Detailed information of TA system TA234461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1385628..1386333
• Replicon: chromosome
• Accession: NZ_CP091677
• Organism: Escherichia coli strain KFu022

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: L6L66_RS06770
• Protein ID: WP_000539521.1
• Coordinates: 1385947..1386333 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L66_RS06765
• Protein ID: WP_001280945.1
• Coordinates: 1385628..1385957 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L66_RS06750 (1380785)	1380785..1381696	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
L6L66_RS06755 (1381874)	1381874..1384222	+	2349	WP_032238219.1	EAL domain-containing protein	-
L6L66_RS06760 (1384230)	1384230..1385558	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
L6L66_RS06765 (1385628)	1385628..1385957	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L66_RS06770 (1385947)	1385947..1386333	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L66_RS06775 (1386559)	1386559..1387884	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L66_RS06780 (1388097)	1388097..1388480	+	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L66_RS06785 (1388591)	1388591..1389706	+	1116	WP_000555031.1	aldose sugar dehydrogenase YliI	-
L6L66_RS06790 (1389703)	1389703..1390329	-	627	WP_016241992.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T234461 WP_000539521.1 NZ_CP091677:c1386333-1385947 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234461 NZ_LR130544:c4002209-4001892 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGTTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGATGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234461 WP_001280945.1 NZ_CP091677:c1385957-1385628 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234461 NZ_LR130544:c4001899-4001612 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6