Detailed information of TA system TA234459

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 815092..815771
• Replicon: chromosome
• Accession: NZ_CP091677
• Organism: Escherichia coli strain KFu022

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: L6L66_RS03995
• Protein ID: WP_000854680.1
• Coordinates: 815092..815433 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: L6L66_RS04000
• Protein ID: WP_000070396.1
• Coordinates: 815454..815771 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L66_RS03970 (810331)	810331..810684	+	354	WP_032238714.1	tail fiber assembly protein	-
L6L66_RS03975 (810716)	810716..811780	-	1065	WP_072003692.1	acyltransferase	-
L6L66_RS03980 (812133)	812133..813044	+	912	WP_073527469.1	IS5 family transposase	-
L6L66_RS03985 (813432)	813432..814175	+	744	WP_032238641.1	AraC family transcriptional regulator	-
L6L66_RS03990 (814217)	814217..814426	-	210	Protein_772	integrase core domain-containing protein	-
L6L66_RS03995 (815092)	815092..815433	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
L6L66_RS04000 (815454)	815454..815771	-	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
L6L66_RS04005 (815790)	815790..816011	-	222	WP_000691994.1	DUF987 domain-containing protein	-
L6L66_RS04010 (816020)	816020..816496	-	477	WP_000811693.1	RadC family protein	-
L6L66_RS04015 (816512)	816512..816970	-	459	WP_000211838.1	antirestriction protein	-
L6L66_RS04020 (817068)	817068..817307	-	240	WP_000194654.1	DUF905 family protein	-
L6L66_RS04025 (817384)	817384..817851	-	468	WP_032238643.1	protein YkfB	-
L6L66_RS04030 (817874)	817874..818317	-	444	WP_000649865.1	hypothetical protein	-
L6L66_RS04035 (818317)	818317..818553	-	237	WP_001144031.1	protein YpjK	-
L6L66_RS04040 (818594)	818594..819295	-	702	WP_000189411.1	WYL domain-containing protein	-
L6L66_RS04045 (819512)	819512..820333	-	822	WP_000197387.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	796759..849088	52329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T234459 WP_000854680.1 NZ_CP091677:c815433-815092 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T234459 NZ_LR130544:1956383-1956485 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT234459 WP_000070396.1 NZ_CP091677:c815771-815454 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT234459 NZ_LR130544:c1956491-1956347 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7