Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yafW-ykfI/CbtA-CbeA |
| Location | 815092..815771 | Replicon | chromosome |
| Accession | NZ_CP091677 | ||
| Organism | Escherichia coli strain KFu022 | ||
Toxin (Protein)
| Gene name | ykfI | Uniprot ID | S1FHS4 |
| Locus tag | L6L66_RS03995 | Protein ID | WP_000854680.1 |
| Coordinates | 815092..815433 (-) | Length | 114 a.a. |
Antitoxin (Protein)
| Gene name | yafW | Uniprot ID | S1EWR7 |
| Locus tag | L6L66_RS04000 | Protein ID | WP_000070396.1 |
| Coordinates | 815454..815771 (-) | Length | 106 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L6L66_RS03970 (810331) | 810331..810684 | + | 354 | WP_032238714.1 | tail fiber assembly protein | - |
| L6L66_RS03975 (810716) | 810716..811780 | - | 1065 | WP_072003692.1 | acyltransferase | - |
| L6L66_RS03980 (812133) | 812133..813044 | + | 912 | WP_073527469.1 | IS5 family transposase | - |
| L6L66_RS03985 (813432) | 813432..814175 | + | 744 | WP_032238641.1 | AraC family transcriptional regulator | - |
| L6L66_RS03990 (814217) | 814217..814426 | - | 210 | Protein_772 | integrase core domain-containing protein | - |
| L6L66_RS03995 (815092) | 815092..815433 | - | 342 | WP_000854680.1 | type IV toxin-antitoxin system toxin YkfI | Toxin |
| L6L66_RS04000 (815454) | 815454..815771 | - | 318 | WP_000070396.1 | type IV toxin-antitoxin system antitoxin YafW | Antitoxin |
| L6L66_RS04005 (815790) | 815790..816011 | - | 222 | WP_000691994.1 | DUF987 domain-containing protein | - |
| L6L66_RS04010 (816020) | 816020..816496 | - | 477 | WP_000811693.1 | RadC family protein | - |
| L6L66_RS04015 (816512) | 816512..816970 | - | 459 | WP_000211838.1 | antirestriction protein | - |
| L6L66_RS04020 (817068) | 817068..817307 | - | 240 | WP_000194654.1 | DUF905 family protein | - |
| L6L66_RS04025 (817384) | 817384..817851 | - | 468 | WP_032238643.1 | protein YkfB | - |
| L6L66_RS04030 (817874) | 817874..818317 | - | 444 | WP_000649865.1 | hypothetical protein | - |
| L6L66_RS04035 (818317) | 818317..818553 | - | 237 | WP_001144031.1 | protein YpjK | - |
| L6L66_RS04040 (818594) | 818594..819295 | - | 702 | WP_000189411.1 | WYL domain-containing protein | - |
| L6L66_RS04045 (819512) | 819512..820333 | - | 822 | WP_000197387.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 796759..849088 | 52329 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 114 a.a. Molecular weight: 12892.95 Da Isoelectric Point: 9.6543
>T234459 WP_000854680.1 NZ_CP091677:c815433-815092 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR
Download Length: 342 bp
>T234459 NZ_LR130544:1956383-1956485 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 106 a.a. Molecular weight: 11982.62 Da Isoelectric Point: 7.0270
>AT234459 WP_000070396.1 NZ_CP091677:c815771-815454 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR
Download Length: 318 bp
>AT234459 NZ_LR130544:c1956491-1956347 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|