Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 3332739..3333331 Replicon chromosome
Accession NC_011757
Organism Methylobacterium extorquens CM4

Toxin (Protein)


Gene name PumA Uniprot ID B7KSA7
Locus tag MCHL_RS15365 Protein ID WP_015951351.1
Coordinates 3332739..3333029 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID B7KSA8
Locus tag MCHL_RS15370 Protein ID WP_015951352.1
Coordinates 3333032..3333331 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCHL_RS15345 3328795..3329751 - 957 WP_015951347.1 hypothetical protein -
MCHL_RS15350 3329726..3330541 - 816 WP_015951348.1 ABC transporter ATP-binding protein -
MCHL_RS15355 3330538..3331578 - 1041 WP_015951349.1 iron ABC transporter permease -
MCHL_RS15360 3331575..3332408 - 834 WP_015951350.1 ABC transporter substrate-binding protein -
MCHL_RS15365 3332739..3333029 + 291 WP_015951351.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MCHL_RS15370 3333032..3333331 + 300 WP_015951352.1 putative addiction module antidote protein Antitoxin
MCHL_RS15375 3333353..3334918 - 1566 WP_015951353.1 glutamine-hydrolyzing GMP synthase -
MCHL_RS15380 3335088..3335315 + 228 WP_003603049.1 hypothetical protein -
MCHL_RS15385 3335501..3336112 - 612 WP_015951354.1 phosphatase PAP2 family protein -
MCHL_RS15390 3336223..3337251 - 1029 WP_015951355.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-87)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 10688.38 Da        Isoelectric Point: 10.4475

>T23445 WP_015951351.1 NC_011757:3332739-3333029 [Methylorubrum extorquens CM4]
MPTIRRTSVFQTWIDDLRDVRAVARIAKRIDRLALGNPGDVKPVGNGVSEMRIDYGPGYRIYFTAQGKQIVILLCGGDKS
SQERDIRAAKALAKEL

Download         Length: 291 bp

>T23445 NC_011757:3332739-3333029 [Methylorubrum extorquens CM4]
ATGCCGACGATCCGCCGTACGAGTGTCTTTCAGACTTGGATCGACGATTTGCGCGACGTTCGCGCCGTTGCGCGCATCGC
CAAGCGCATCGACCGGTTGGCGCTCGGCAATCCGGGCGACGTCAAACCGGTCGGAAACGGTGTCAGCGAGATGCGGATCG
ATTACGGCCCCGGCTACCGGATCTACTTCACCGCCCAAGGCAAGCAGATCGTCATTTTGCTTTGCGGCGGAGACAAGAGC
TCACAGGAGCGGGACATCCGAGCTGCGAAAGCGCTCGCGAAGGAGCTTTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.34 Da        Isoelectric Point: 5.5368

>AT23445 WP_015951352.1 NC_011757:3333032-3333331 [Methylorubrum extorquens CM4]
MALETQPYDSAEHLKTPEHIAAYLDAVLEDNDPALLAHALGVIARARGMTQIAQDVGRSREQLYRTLSDKGNPQLDTLMG
VLKSLGLRLSVHPVDVERS

Download         Length: 300 bp

>AT23445 NC_011757:3333032-3333331 [Methylorubrum extorquens CM4]
ATGGCCCTCGAAACGCAGCCTTACGACAGCGCCGAGCACCTGAAGACGCCCGAACACATCGCGGCCTATCTCGATGCCGT
GTTGGAAGATAACGATCCCGCACTGCTCGCTCACGCGCTCGGTGTCATCGCGCGCGCCCGCGGAATGACGCAGATCGCTC
AGGACGTCGGCCGGTCGCGAGAACAACTCTACCGAACCCTGTCCGACAAAGGGAACCCGCAACTCGACACGCTCATGGGT
GTGCTGAAGTCGTTAGGATTGCGCCTCAGCGTGCATCCGGTCGACGTAGAGCGATCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B7KSA7

Antitoxin

Source ID Structure
AlphaFold DB B7KSA8

References