Detailed information of TA system TA234445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4315537..4316168
• Replicon: chromosome
• Accession: NZ_CP091673
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: L6L68_RS22045
• Protein ID: WP_001260301.1
• Coordinates: 4315893..4316168 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: L6L68_RS22040
• Protein ID: WP_000593557.1
• Coordinates: 4315537..4315896 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS22015 (4311669)	4311669..4312613	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
L6L68_RS22020 (4312610)	4312610..4313443	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
L6L68_RS22025 (4313443)	4313443..4314207	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
L6L68_RS22030 (4314204)	4314204..4315010	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
L6L68_RS22035 (4315016)	4315016..4315417	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
L6L68_RS22040 (4315537)	4315537..4315896	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L6L68_RS22045 (4315893)	4315893..4316168	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
L6L68_RS22050 (4316241)	4316241..4317365	-	1125	WP_001318088.1	ABC transporter permease	-
L6L68_RS22055 (4317365)	4317365..4320100	-	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
L6L68_RS22060 (4320097)	4320097..4321164	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T234445 WP_001260301.1 NZ_CP091673:c4316168-4315893 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T234445 NZ_LR130543:762948-763358 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT234445 WP_000593557.1 NZ_CP091673:c4315896-4315537 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT234445 NZ_LR130543:762701-762967 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1