Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3671956..3672610 Replicon chromosome
Accession NZ_CP091673
Organism Escherichia coli strain KKo009

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag L6L68_RS18780 Protein ID WP_000244781.1
Coordinates 3671956..3672363 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L68_RS18785 Protein ID WP_000354046.1
Coordinates 3672344..3672610 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L68_RS18760 (3667913) 3667913..3669646 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
L6L68_RS18765 (3669652) 3669652..3670362 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L68_RS18770 (3670387) 3670387..3671283 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L68_RS18775 (3671395) 3671395..3671916 + 522 WP_001055874.1 flavodoxin FldB -
L6L68_RS18780 (3671956) 3671956..3672363 - 408 WP_000244781.1 protein YgfX Toxin
L6L68_RS18785 (3672344) 3672344..3672610 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L68_RS18790 (3672863) 3672863..3673843 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
L6L68_RS18795 (3673920) 3673920..3674579 - 660 WP_000250269.1 hemolysin III family protein -
L6L68_RS18800 (3674743) 3674743..3675054 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
L6L68_RS18805 (3675099) 3675099..3676532 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T234441 WP_000244781.1 NZ_CP091673:c3672363-3671956 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234441 NZ_LR130542:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234441 WP_000354046.1 NZ_CP091673:c3672610-3672344 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234441 NZ_LR130542:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References