Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2744022..2744670 Replicon chromosome
Accession NC_011757
Organism Methylobacterium extorquens CM4

Toxin (Protein)


Gene name hicA Uniprot ID B7L2G2
Locus tag MCHL_RS12640 Protein ID WP_015950975.1
Coordinates 2744022..2744225 (+) Length 68 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B7L2G3
Locus tag MCHL_RS12645 Protein ID WP_015950976.1
Coordinates 2744263..2744670 (+) Length 136 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MCHL_RS12620 2739499..2740014 + 516 WP_015950972.1 signal peptidase II -
MCHL_RS12625 2740177..2740881 + 705 WP_015950973.1 hypothetical protein -
MCHL_RS12630 2741074..2742456 + 1383 WP_003599161.1 insulinase family protein -
MCHL_RS12635 2742528..2743811 + 1284 WP_015950974.1 insulinase family protein -
MCHL_RS12640 2744022..2744225 + 204 WP_015950975.1 type II toxin-antitoxin system HicA family toxin Toxin
MCHL_RS12645 2744263..2744670 + 408 WP_015950976.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MCHL_RS12650 2744753..2745685 + 933 WP_015950977.1 ring-cleaving dioxygenase -
MCHL_RS12655 2745855..2746628 + 774 WP_003599169.1 ferredoxin--NADP reductase -
MCHL_RS12660 2746923..2747600 + 678 WP_015950978.1 dethiobiotin synthase -
MCHL_RS12665 2747607..2747966 - 360 WP_003599173.1 hypothetical protein -
MCHL_RS12670 2748001..2748420 - 420 WP_003599175.1 response regulator -
MCHL_RS12675 2748688..2749362 + 675 WP_015822797.1 DUF1275 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-65)

Antitoxin

(8-126)


Sequences


Toxin        


Download         Length: 68 a.a.        Molecular weight: 7724.78 Da        Isoelectric Point: 11.7277

>T23444 WP_015950975.1 NC_011757:2744022-2744225 [Methylorubrum extorquens CM4]
VSNRAKETRSLIVRLRDDGWIVARKGPGDHVQYKHPTKPGRVTLDTGRREQALGTLRSIFRQAGWEW

Download         Length: 204 bp

>T23444 NC_011757:2744022-2744225 [Methylorubrum extorquens CM4]
GTGTCGAATCGCGCCAAGGAAACGCGCAGCCTCATAGTCCGACTGCGCGATGATGGTTGGATCGTTGCCCGGAAGGGGCC
GGGCGACCATGTCCAGTACAAACATCCAACGAAACCGGGACGTGTGACGCTGGATACCGGGCGCCGCGAGCAGGCGCTGG
GTACGCTCCGCAGCATCTTCAGGCAGGCGGGGTGGGAATGGTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14531.49 Da        Isoelectric Point: 4.4886

>AT23444 WP_015950976.1 NC_011757:2744263-2744670 [Methylorubrum extorquens CM4]
MTRVVMLIHGEAGNYGASFPDFPGATTGADDLDELYRKAAEMLAFHVGGLVEDGELVPRTRSLEELRGDPVFIEESRDAM
IGLIEVDLPGRAVRVNISVEESLLKRIDRAAEAAGESRSGFLAQAAKARLTSMVA

Download         Length: 408 bp

>AT23444 NC_011757:2744263-2744670 [Methylorubrum extorquens CM4]
ATGACGCGGGTCGTCATGCTGATTCACGGCGAAGCCGGCAATTACGGCGCGTCTTTCCCGGATTTTCCCGGTGCGACGAC
GGGCGCCGACGATCTCGACGAGCTCTACCGGAAGGCCGCCGAGATGCTGGCCTTCCATGTCGGCGGCTTGGTGGAGGATG
GCGAACTGGTGCCGCGGACCCGCTCGCTTGAAGAACTGCGCGGCGACCCCGTTTTCATTGAGGAAAGCCGGGACGCGATG
ATCGGCCTGATCGAGGTCGATCTCCCGGGGCGTGCCGTGCGGGTGAACATCTCGGTCGAGGAGAGTCTGCTCAAGCGGAT
CGACCGCGCGGCCGAGGCCGCCGGCGAGAGCCGATCCGGCTTCCTCGCGCAGGCCGCGAAGGCGCGCCTGACGTCCATGG
TGGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB B7L2G2


Antitoxin

Source ID Structure
AlphaFold DB B7L2G3

References