Detailed information of TA system TA023443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1852334..1852869
• Replicon: chromosome
• Accession: NC_011757
• Organism: Methylobacterium extorquens CM4

Toxin (Protein)

• Gene name: parE
• Uniprot ID: B7KUF4
• Locus tag: MCHL_RS08655
• Protein ID: WP_015950446.1
• Coordinates: 1852579..1852869 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: MCHL_RS08650
• Protein ID: WP_012253127.1
• Coordinates: 1852334..1852582 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCHL_RS08635	1847552..1849135	-	1584	WP_015950444.1	hypothetical protein	-
MCHL_RS08640	1849135..1850502	-	1368	WP_015950445.1	SWIM zinc finger family protein	-
MCHL_RS08645	1850698..1852200	-	1503	WP_012752481.1	glucose-6-phosphate dehydrogenase	-
MCHL_RS08650	1852334..1852582	+	249	WP_012253127.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
MCHL_RS08655	1852579..1852869	+	291	WP_015950446.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MCHL_RS08660	1852951..1853589	+	639	WP_041928975.1	uracil-DNA glycosylase family protein	-
MCHL_RS08665	1853597..1855123	-	1527	WP_015950448.1	alginate export family protein	-
MCHL_RS08670	1855448..1856146	+	699	WP_015950449.1	orotidine-5'-phosphate decarboxylase	-
MCHL_RS08675	1856149..1856493	+	345	WP_003605408.1	DUF1330 domain-containing protein	-
MCHL_RS08680	1856513..1857136	-	624	WP_012253132.1	TetR/AcrR family transcriptional regulator	-
MCHL_RS08685	1857359..1857559	-	201	WP_003605412.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11039.61 Da
• Isoelectric Point: 10.5105

>T23443 WP_015950446.1 NC_011757:1852579-1852869 [Methylorubrum extorquens CM4]
MSVRLSPRARADLSRIWDDSAERWGADQADRYIRLLAGGFDRLAEDPARGLRADEIRKGYFRLSVGSHILFYRLGAEGGI
EVIRILHGRMDFKRHL

Nucleotide

• Length: 291 bp

>T23443 NC_011757:1852579-1852869 [Methylorubrum extorquens CM4]
ATGAGCGTCCGCCTCTCGCCGAGGGCGCGCGCGGATCTCAGCCGGATCTGGGACGATTCGGCCGAGCGCTGGGGAGCGGA
TCAGGCCGACCGCTACATCCGCCTTCTGGCTGGAGGCTTCGACCGATTGGCGGAGGATCCGGCTCGAGGTCTGAGGGCCG
ATGAGATCCGCAAGGGGTATTTCCGCCTCTCCGTGGGATCGCACATCCTGTTCTACCGCCTGGGCGCCGAGGGCGGCATC
GAGGTCATCCGCATCCTTCACGGGCGGATGGATTTCAAACGCCATCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8974.00 Da
• Isoelectric Point: 4.5173

>AT23443 WP_012253127.1 NC_011757:1852334-1852582 [Methylorubrum extorquens CM4]
MPRNTSVSLGEHFAQFIDRQVEGGRYGSASEVVRAGLRLLEEQEAALDTLRAALIEGEESGPPELFDTEAFLREKRAGAI
RG

Nucleotide

• Length: 249 bp

>AT23443 NC_011757:1852334-1852582 [Methylorubrum extorquens CM4]
ATGCCGCGAAACACGTCCGTCTCGCTCGGCGAGCACTTTGCGCAGTTCATCGACCGCCAGGTCGAGGGGGGACGTTACGG
CTCCGCCAGCGAAGTGGTCCGGGCCGGGCTTCGTCTCCTTGAGGAGCAGGAGGCCGCCCTCGACACGCTCCGGGCCGCGC
TGATCGAGGGGGAGGAGAGCGGCCCGCCCGAGCTCTTCGATACGGAGGCCTTTCTCCGGGAGAAGCGTGCGGGCGCGATC
CGAGGATGA

Structures

Toxin

• AlphaFold DB: B7KUF4