Detailed information of TA system TA234429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2029297..2029933
• Replicon: chromosome
• Accession: NZ_CP091673
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: L6L68_RS10925
• Protein ID: WP_000813796.1
• Coordinates: 2029297..2029473 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L6L68_RS10930
• Protein ID: WP_076838470.1
• Coordinates: 2029517..2029933 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS10905 (2024919)	2024919..2026091	-	1173	WP_001236216.1	BenE family transporter YdcO	-
L6L68_RS10910 (2026183)	2026183..2026719	+	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
L6L68_RS10915 (2026792)	2026792..2028753	+	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L6L68_RS10920 (2028845)	2028845..2029075	-	231	WP_000494243.1	YncJ family protein	-
L6L68_RS10925 (2029297)	2029297..2029473	+	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L6L68_RS10930 (2029517)	2029517..2029933	+	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L6L68_RS10935 (2030012)	2030012..2031418	+	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
L6L68_RS10940 (2031663)	2031663..2032808	+	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
L6L68_RS10945 (2032826)	2032826..2033839	+	1014	WP_000220433.1	ABC transporter ATP-binding protein	-
L6L68_RS10950 (2033840)	2033840..2034781	+	942	WP_001251307.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T234429 WP_000813796.1 NZ_CP091673:2029297-2029473 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T234429 NZ_LR130541:c89469-89149 [Klebsiella pneumoniae]
ATGAAAACTCAACCTGCAACAACTCCGCAGGCGGCGAAGCCATGTCTGTCGCCCCTGGCTGTCTGGCAAATGTTACTGAC
AAGCCTGCTAGACCAGCACTATGGTCTCACGCTAAACGACACGCCGTTCAGTGATGAAACTATTATTCAGAAACACATTG
AGGCAGGTATTACCTTGGCCGATGCCGTAAACTTTCTGGTTGAACGCTATGAACTAGTTCGTATTGACCAAAAAGGCTTC
TCTGTGCAAGATCAAGAACCTTGGTTAACCTCTATGGATGTTCACCGAGCACGATTTAAACTCAACGTGGAACGTTTATA
A

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT234429 WP_076838470.1 NZ_CP091673:2029517-2029933 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT234429 NZ_LR130541:c89816-89490 [Klebsiella pneumoniae]
ATGTCATTAATACCAGCCCATGAATGGGGACTACAAAGAGAAATTGTTCCACGGTTTGGTGCCAGATTAGTTCAGGAGGG
CAACCGGCTGCATTATCTGGCTGACAGGGCTAATTTGATGGGCAATTTCAGCGATACGGAATGCTTCAAATTGAATGACG
CATTTCCACACTTTATAAGTCAGATGGAATCGATGCTGACCACTGGTGAACTAATCCCCTGTCACCATCACTGTGTCACT
CTCTACCACAACGGTTTTACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTGTACATCGCGGTTTATCCCACTCA
ACGCTAA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3