Detailed information of TA system TA234428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1722295..1723079
• Replicon: chromosome
• Accession: NZ_CP091673
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: L6L68_RS09380
• Protein ID: WP_000613624.1
• Coordinates: 1722295..1722789 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: L6L68_RS09385
• Protein ID: WP_001110446.1
• Coordinates: 1722786..1723079 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS09360 (1717488)	1717488..1718585	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
L6L68_RS09365 (1718585)	1718585..1719526	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
L6L68_RS09370 (1719592)	1719592..1721235	+	1644	WP_000096482.1	flagellar hook-associated protein FlgK	-
L6L68_RS09375 (1721247)	1721247..1722200	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
L6L68_RS09380 (1722295)	1722295..1722789	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
L6L68_RS09385 (1722786)	1722786..1723079	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
L6L68_RS09390 (1723213)	1723213..1726398	-	3186	WP_000827395.1	ribonuclease E	-
L6L68_RS09395 (1726971)	1726971..1727930	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T234428 WP_000613624.1 NZ_CP091673:c1722789-1722295 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T234428 NZ_LR130540:53878-54360 [Klebsiella variicola]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCCGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGTGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT234428 WP_001110446.1 NZ_CP091673:c1723079-1722786 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT234428 NZ_LR130540:53624-53890 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCGCCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A829G775