Detailed information of TA system TA234427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1475015..1475720
• Replicon: chromosome
• Accession: NZ_CP091673
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: L6L68_RS08190
• Protein ID: WP_000539521.1
• Coordinates: 1475334..1475720 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L68_RS08185
• Protein ID: WP_001280945.1
• Coordinates: 1475015..1475344 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS08170 (1470172)	1470172..1471083	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
L6L68_RS08175 (1471261)	1471261..1473609	+	2349	Protein_1362	EAL domain-containing protein	-
L6L68_RS08180 (1473617)	1473617..1474945	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
L6L68_RS08185 (1475015)	1475015..1475344	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L68_RS08190 (1475334)	1475334..1475720	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L68_RS08195 (1475946)	1475946..1477271	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L68_RS08200 (1477484)	1477484..1477867	+	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L68_RS08205 (1477978)	1477978..1479093	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
L6L68_RS08210 (1479090)	1479090..1479716	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T234427 WP_000539521.1 NZ_CP091673:c1475720-1475334 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234427 NZ_LR130540:7886-8302 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234427 WP_001280945.1 NZ_CP091673:c1475344-1475015 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234427 NZ_LR130540:7659-7889 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6