Detailed information of TA system TA234424

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 801297..802095
• Replicon: chromosome
• Accession: NZ_CP091673
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: L6L68_RS04995
• Protein ID: WP_000854730.1
• Coordinates: 801297..801674 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: L6L68_RS05000
• Protein ID: WP_001285481.1
• Coordinates: 801721..802095 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS04965 (797095)	797095..797289	-	195	WP_001313570.1	DUF957 domain-containing protein	-
L6L68_RS04970 (797331)	797331..797622	+	292	Protein_734	transposase	-
L6L68_RS04975 (797619)	797619..797969	+	351	WP_000624707.1	IS66 family insertion sequence element accessory protein TnpB	-
L6L68_RS04980 (798000)	798000..799593	+	1594	Protein_736	IS66 family transposase	-
L6L68_RS04985 (799741)	799741..800729	+	989	Protein_737	IS630 family transposase	-
L6L68_RS04990 (800808)	800808..801300	-	493	Protein_738	DUF5983 family protein	-
L6L68_RS04995 (801297)	801297..801674	-	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
L6L68_RS05000 (801721)	801721..802095	-	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L6L68_RS05005 (802145)	802145..802789	-	645	WP_000086759.1	hypothetical protein	-
L6L68_RS05010 (802808)	802808..803029	-	222	WP_000692315.1	DUF987 domain-containing protein	-
L6L68_RS05015 (803092)	803092..803568	-	477	WP_001313574.1	RadC family protein	-
L6L68_RS05020 (803584)	803584..804057	-	474	WP_001313575.1	antirestriction protein	-
L6L68_RS05025 (804151)	804151..804396	-	246	WP_001164966.1	hypothetical protein	-
L6L68_RS05030 (804396)	804396..805217	-	822	WP_001234359.1	DUF932 domain-containing protein	-
L6L68_RS05035 (805317)	805317..805550	-	234	WP_001213776.1	DUF905 family protein	-
L6L68_RS05040 (805668)	805668..806120	-	453	WP_000682723.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T234424 WP_000854730.1 NZ_CP091673:c801674-801297 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T234424 NZ_LR130539:c4943566-4943282 [Klebsiella variicola]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT234424 WP_001285481.1 NZ_CP091673:c802095-801721 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT234424 NZ_LR130539:c4943798-4943556 [Klebsiella variicola]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGACTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8