Detailed information of TA system TA234422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 216245..216804
• Replicon: plasmid pKKo009_1
• Accession: NZ_CP091672
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L6L68_RS01145
• Protein ID: WP_001620845.1
• Coordinates: 216529..216804 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: L6L68_RS01140
• Protein ID: WP_001178089.1
• Coordinates: 216245..216529 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS01120 (211336)	211336..211593	+	258	WP_164999210.1	PapB/FocB family fimbrial expression transcriptional regulator	-
L6L68_RS01125 (212157)	212157..212381	-	225	Protein_228	IS3 family transposase	-
L6L68_RS01130 (212407)	212407..215373	-	2967	WP_000008826.1	Tn3 family transposase	-
L6L68_RS01135 (215354)	215354..215977	-	624	WP_000904945.1	recombinase family protein	-
L6L68_RS01140 (216245)	216245..216529	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
L6L68_RS01145 (216529)	216529..216804	+	276	WP_001620845.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L68_RS01150 (216910)	216910..217113	+	204	Protein_233	hypothetical protein	-
L6L68_RS01155 (217384)	217384..218561	-	1178	Protein_234	IS91 family transposase	-
L6L68_RS01160 (218561)	218561..218926	-	366	WP_000124086.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1A / floR / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	espC / iroN / iroE / iroD / iroC / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..224424	224424
	-	inside	IScluster/Tn	-	-	212157..218561	6404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10763.47 Da
• Isoelectric Point: 9.9324

>T234422 WP_001620845.1 NZ_CP091672:216529-216804 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREIRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T234422 NZ_LR130539:c4053135-4052818 [Klebsiella variicola]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAAATAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT234422 WP_001178089.1 NZ_CP091672:216245-216529 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT234422 NZ_LR130539:c4052825-4052538 [Klebsiella variicola]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGTGGAATAAAGCACCCACAAATTTTACTTTACTAA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8