Detailed information of TA system TA234419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 29794..30316
• Replicon: plasmid pKKo009_1
• Accession: NZ_CP091672
• Organism: Escherichia coli strain KKo009

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: L6L68_RS00140
• Protein ID: WP_000220560.1
• Coordinates: 30035..30316 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: L6L68_RS00135
• Protein ID: WP_000121743.1
• Coordinates: 29794..30045 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L68_RS00105 (24794)	24794..25456	+	663	WP_000516402.1	division plane positioning ATPase MipZ	-
L6L68_RS00110 (25556)	25556..25840	+	285	WP_001185480.1	hypothetical protein	-
L6L68_RS00115 (26275)	26275..26871	+	597	WP_058678168.1	YcxB family protein	-
L6L68_RS00120 (26923)	26923..27279	-	357	WP_096488222.1	DNA distortion polypeptide 3	-
L6L68_RS00125 (27416)	27416..27862	-	447	WP_021532249.1	hypothetical protein	-
L6L68_RS00130 (27887)	27887..28741	-	855	WP_001239324.1	replication initiation protein	-
L6L68_RS00135 (29794)	29794..30045	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
L6L68_RS00140 (30035)	30035..30316	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L68_RS00145 (30462)	30462..30785	+	324	WP_001181903.1	hypothetical protein	-
L6L68_RS00150 (30830)	30830..31075	+	246	WP_000356546.1	hypothetical protein	-
L6L68_RS00155 (31065)	31065..31280	+	216	WP_001180116.1	hypothetical protein	-
L6L68_RS00160 (31669)	31669..32846	-	1178	Protein_33	IS91 family transposase	-
L6L68_RS00165 (32846)	32846..33211	-	366	WP_000124086.1	hypothetical protein	-
L6L68_RS00170 (33293)	33293..33895	+	603	Protein_35	IS3-like element IS2 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / blaTEM-1B / aadA5 / qacE / sul1 / mph(A) / erm(B) / aac(3)-IId / blaTEM-1A / floR / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A)	espC / iroN / iroE / iroD / iroC / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..224424	224424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T234419 WP_000220560.1 NZ_CP091672:30035-30316 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T234419 NZ_LR130539:1792343-1792675 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT234419 WP_000121743.1 NZ_CP091672:29794-30045 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT234419 NZ_LR130539:1792085-1792342 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301