Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 151463..151988 Replicon plasmid pKOr019_1
Accession NZ_CP091666
Organism Escherichia coli strain KOr019

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag L6L58_RS25750 Protein ID WP_001159868.1
Coordinates 151683..151988 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag L6L58_RS25745 Protein ID WP_000813634.1
Coordinates 151463..151681 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L58_RS25715 (146540) 146540..146887 + 348 WP_000142449.1 DUF6404 family protein -
L6L58_RS25720 (146907) 146907..147497 - 591 WP_000194553.1 hypothetical protein -
L6L58_RS25725 (147497) 147497..147754 - 258 WP_000371889.1 hypothetical protein -
L6L58_RS25730 (148082) 148082..149185 + 1104 WP_001127573.1 nucleotide-binding protein -
L6L58_RS25735 (149215) 149215..150345 + 1131 WP_000542418.1 DUF4238 domain-containing protein -
L6L58_RS25740 (150345) 150345..150635 + 291 WP_001229374.1 hypothetical protein -
L6L58_RS25745 (151463) 151463..151681 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L6L58_RS25750 (151683) 151683..151988 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
L6L58_RS25755 (151989) 151989..152357 + 369 Protein_164 tyrosine-type recombinase/integrase -
L6L58_RS25760 (152528) 152528..153640 - 1113 WP_001300563.1 IS4-like element IS421 family transposase -
L6L58_RS25765 (153695) 153695..154144 + 450 Protein_166 tyrosine-type recombinase/integrase -
L6L58_RS25770 (154324) 154324..154968 + 645 WP_001144036.1 ParA family protein -
L6L58_RS25775 (155055) 155055..155363 + 309 WP_000030198.1 hypothetical protein -
L6L58_RS25785 (156329) 156329..156691 + 363 Protein_170 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..158848 158848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T234418 WP_001159868.1 NZ_CP091666:151683-151988 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T234418 NZ_LR130539:806207-806617 [Klebsiella variicola]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGTTGGTTCCCTGGCCGCTAAGCTACACCCCGATCTGGCTGCTGCTGTTGTCGCTGGTAGTGTTTGACTGCG
TACGCAGCCAGCGGCGGATCCATGCCCGTCGCGGAGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTCATCAATAGCGGTATGCTGCTGCGTTTACGCCATGTCGATACCCGGCGCGG
GCAGCATCTGTGGCTGGCGGCGGATAGCATGGATGCCGGAGAATGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT234418 WP_000813634.1 NZ_CP091666:151463-151681 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT234418 NZ_LR130539:805960-806226 [Klebsiella variicola]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References