Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4996640..4997256 Replicon chromosome
Accession NZ_CP091665
Organism Escherichia coli strain KOr019

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag L6L58_RS23875 Protein ID WP_001129490.1
Coordinates 4996640..4997014 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag L6L58_RS23880 Protein ID WP_001275523.1
Coordinates 4997014..4997256 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L58_RS23860 (4994143) 4994143..4995045 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
L6L58_RS23865 (4995042) 4995042..4995677 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
L6L58_RS23870 (4995674) 4995674..4996603 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
L6L58_RS23875 (4996640) 4996640..4997014 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
L6L58_RS23880 (4997014) 4997014..4997256 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
L6L58_RS23885 (4997476) 4997476..4997694 - 219 WP_001251290.1 CopG family transcriptional regulator -
L6L58_RS23890 (4998368) 4998368..4999282 - 915 WP_072649161.1 transposase -
L6L58_RS23895 (4999295) 4999295..5000182 - 888 Protein_4691 hypothetical protein -
L6L58_RS23900 (5000598) 5000598..5001539 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
L6L58_RS23905 (5001584) 5001584..5002021 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T234409 WP_001129490.1 NZ_CP091665:c4997014-4996640 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T234409 NZ_LR130538:1766715-1767047 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTTAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTCGTGGTTCCCGTGACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGTAAGCGTCTGGAACGCATACCTGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT234409 WP_001275523.1 NZ_CP091665:c4997256-4997014 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT234409 NZ_LR130538:1766457-1766714 [Klebsiella variicola]
ATGTATAAAACTCGTCTTAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTTAAAACGCTGGAGCT
ATCTACGGACAGCGAAGTCGGCATGACCATTAACAATGGATGCCTGATTATCGAACCCCAGAAACGACCTCATTATTCGC
TGGAGGAGCTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGAAATGGATTGATGCGCCTGCGATG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References