Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4375315..4375891 Replicon chromosome
Accession NZ_CP091665
Organism Escherichia coli strain KOr019

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag L6L58_RS21005 Protein ID WP_001131965.1
Coordinates 4375604..4375891 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag L6L58_RS21000 Protein ID WP_000063158.1
Coordinates 4375315..4375617 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L58_RS20980 (4370423) 4370423..4371643 + 1221 WP_000873756.1 amino acid deaminase -
L6L58_RS20985 (4371911) 4371911..4374061 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
L6L58_RS20990 (4374111) 4374111..4374314 + 204 WP_000467859.1 YbdD/YjiX family protein -
L6L58_RS20995 (4374325) 4374325..4375281 + 957 WP_001361361.1 GTPase -
L6L58_RS21000 (4375315) 4375315..4375617 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
L6L58_RS21005 (4375604) 4375604..4375891 - 288 WP_001131965.1 BrnT family toxin Toxin
L6L58_RS21010 (4376088) 4376088..4377254 + 1167 WP_000800823.1 restriction endonuclease -
L6L58_RS21015 (4377299) 4377299..4377472 + 174 Protein_4131 type I restriction-modification system subunit M N-terminal domain-containing protein -
L6L58_RS21020 (4377466) 4377466..4378134 + 669 Protein_4132 DUF3387 domain-containing protein -
L6L58_RS21025 (4378349) 4378349..4378687 + 339 WP_000132633.1 endoribonuclease SymE -
L6L58_RS21030 (4378775) 4378775..4378822 - 48 WP_229017048.1 hypothetical protein -
L6L58_RS21035 (4379070) 4379070..4380482 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T234404 WP_001131965.1 NZ_CP091665:c4375891-4375604 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T234404 NZ_LR130537:c5391824-5391546 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT234404 WP_000063158.1 NZ_CP091665:c4375617-4375315 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT234404 NZ_LR130537:c5391534-5391229 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References