Detailed information of TA system TA234400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3410243..3410948
• Replicon: chromosome
• Accession: NZ_CP091665
• Organism: Escherichia coli strain KOr019

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: L6L58_RS16265
• Protein ID: WP_000539524.1
• Coordinates: 3410243..3410629 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L58_RS16270
• Protein ID: WP_001280945.1
• Coordinates: 3410619..3410948 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L58_RS16245 (3406247)	3406247..3406873	+	627	WP_001295292.1	glutathione S-transferase GstB	-
L6L58_RS16250 (3406870)	3406870..3407985	-	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
L6L58_RS16255 (3408096)	3408096..3408479	-	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L58_RS16260 (3408692)	3408692..3410017	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L58_RS16265 (3410243)	3410243..3410629	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L58_RS16270 (3410619)	3410619..3410948	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L58_RS16275 (3411018)	3411018..3412346	-	1329	WP_167824314.1	GGDEF domain-containing protein	-
L6L58_RS16280 (3412354)	3412354..3414702	-	2349	WP_000950331.1	EAL domain-containing protein	-
L6L58_RS16285 (3414879)	3414879..3415790	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T234400 WP_000539524.1 NZ_CP091665:3410243-3410629 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234400 NZ_LR130537:c141819-141538 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAAGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234400 WP_001280945.1 NZ_CP091665:3410619-3410948 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234400 NZ_LR130537:c142043-141816 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7