Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 166729..167341 Replicon chromosome
Accession NZ_CP091665
Organism Escherichia coli strain KOr019

Toxin (Protein)


Gene name hicA Uniprot ID U9YXE2
Locus tag L6L58_RS00720 Protein ID WP_000833473.1
Coordinates 166729..166914 (+) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1M2IVK0
Locus tag L6L58_RS00725 Protein ID WP_000499742.1
Coordinates 166931..167341 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L58_RS00705 (162195) 162195..163490 + 1296 WP_000985745.1 Fic family protein -
L6L58_RS00710 (163598) 163598..165136 + 1539 WP_000183978.1 aldehyde dehydrogenase AldB -
L6L58_RS00715 (165177) 165177..166256 - 1080 WP_000061475.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
L6L58_RS00720 (166729) 166729..166914 + 186 WP_000833473.1 type II toxin-antitoxin system HicA family toxin Toxin
L6L58_RS00725 (166931) 166931..167341 + 411 WP_000499742.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
L6L58_RS00730 (167453) 167453..169432 - 1980 WP_001026863.1 glycoside hydrolase family 127 protein -
L6L58_RS00735 (169443) 169443..170843 - 1401 WP_000204814.1 MFS transporter -
L6L58_RS00740 (171069) 171069..171884 + 816 WP_000891820.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6800.89 Da        Isoelectric Point: 11.7053

>T234381 WP_000833473.1 NZ_CP091665:166729-166914 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T234381 NZ_LR130534:c143682-143401 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15242.13 Da        Isoelectric Point: 4.4482

>AT234381 WP_000499742.1 NZ_CP091665:166931-167341 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT234381 NZ_LR130534:c143906-143679 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9YXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IVK0

References