Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4760321..4760818 Replicon chromosome
Accession NC_011750
Organism Escherichia coli IAI39

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECIAI39_RS22850 Protein ID WP_001173343.1
Coordinates 4760645..4760818 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag ECIAI39_RS22845 Protein ID WP_000398619.1
Coordinates 4760321..4760617 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI39_RS22825 4757050..4757769 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECIAI39_RS22830 4757766..4759397 - 1632 WP_001216448.1 sensor histidine kinase -
ECIAI39_RS22835 4759578..4759808 + 231 WP_000371713.1 (4Fe-4S)-binding protein -
ECIAI39_RS22840 4759820..4760092 + 273 WP_000405647.1 N-acetyltransferase -
ECIAI39_RS22845 4760321..4760617 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECIAI39_RS22850 4760645..4760818 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECIAI39_RS22855 4760937..4762454 - 1518 WP_000003800.1 lysine--tRNA ligase -
ECIAI39_RS22860 4762691..4764143 - 1453 Protein_4465 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T23438 WP_001173343.1 NC_011750:4760645-4760818 [Escherichia coli IAI39]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T23438 NC_011750:4760645-4760818 [Escherichia coli IAI39]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT23438 WP_000398619.1 NC_011750:4760321-4760617 [Escherichia coli IAI39]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT23438 NC_011750:4760321-4760617 [Escherichia coli IAI39]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References